Next Article in Journal
Polymorphisms in DNA Repair Genes and MDR1 and the Risk for Non-Hodgkin Lymphoma
Next Article in Special Issue
Biomarkers in Alzheimer’s Disease Analysis by Mass Spectrometry-Based Proteomics
Previous Article in Journal
Proteomic Analysis of Etiolated Juvenile Tetraploid Robinia pseudoacacia Branches during Different Cutting Periods
Previous Article in Special Issue
Quality Control and Stability Studies with the Monoclonal Antibody, Trastuzumab: Application of 1D- vs. 2D-Gel Electrophoresis
Article Menu

Export Article

Open AccessArticle

Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase

Department Microbiology and Parasitology, Faculty Pharmacy, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain
Department Microbiology, Faculty Biology, University Barcelona, Diagonal 643, 08071 Barcelona, Spain
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia
Author to whom correspondence should be addressed.
In memoriam.
Int. J. Mol. Sci. 2014, 15(4), 6689-6702;
Received: 3 January 2014 / Revised: 3 March 2014 / Accepted: 9 April 2014 / Published: 21 April 2014
(This article belongs to the Collection Advances in Proteomic Research)
PDF [1471 KB, uploaded 19 June 2014]


By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains. View Full-Text
Keywords: Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Aquilini, E.; Merino, S.; Knirel, Y.A.; Regué, M.; Tomás, J.M. Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase. Int. J. Mol. Sci. 2014, 15, 6689-6702.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top