Next Article in Journal
Genetic Deletion of Rheb1 in the Brain Reduces Food Intake and Causes Hypoglycemia with Altered Peripheral Metabolism
Next Article in Special Issue
Genetic Variants of GPER/GPR30, a Novel Estrogen-Related G Protein Receptor, Are Associated with Human Seminoma
Previous Article in Journal
Plant Dependence on Rhizobia for Nitrogen Influences Induced Plant Defenses and Herbivore Performance
Previous Article in Special Issue
G Protein-Coupled Receptors: What a Difference a ‘Partner’ Makes
Open AccessArticle

Dopamine D4 Receptor Counteracts Morphine-Induced Changes in µ Opioid Receptor Signaling in the Striosomes of the Rat Caudate Putamen

1
Department of Cell Biology, School of Science, University of Málaga, 29071 Málaga, Spain
2
Department of Neuroscience, Biodonostia Institute, 20014 San Sebastián, Spain
3
Laboratory of Eukaryotic Gene Expression and Signal Transduction (LEGEST), Ghent University-Gent, 9000 Ghent, Belgium
4
Department of Neuroscience, Karolinska Institutet, Retzius väg 8, 17177 Stockholm, Sweden
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(1), 1481-1498; https://doi.org/10.3390/ijms15011481
Received: 30 November 2013 / Revised: 8 January 2014 / Accepted: 13 January 2014 / Published: 21 January 2014
(This article belongs to the Collection G Protein-Coupled Receptor Signaling and Regulation)
The mu opioid receptor (MOR) is critical in mediating morphine analgesia. However, prolonged exposure to morphine induces adaptive changes in this receptor leading to the development of tolerance and addiction. In the present work we have studied whether the continuous administration of morphine induces changes in MOR protein levels, its pharmacological profile, and MOR-mediated G-protein activation in the striosomal compartment of the rat CPu, by using immunohistochemistry and receptor and DAMGO-stimulated [35S]GTPγS autoradiography. MOR immunoreactivity, agonist binding density and its coupling to G proteins are up-regulated in the striosomes by continuous morphine treatment in the absence of changes in enkephalin and dynorphin mRNA levels. In addition, co-treatment of morphine with the dopamine D4 receptor (D4R) agonist PD168,077 fully counteracts these adaptive changes in MOR, in spite of the fact that continuous PD168,077 treatment increases the [3H]DAMGO Bmax values to the same degree as seen after continuous morphine treatment. Thus, in spite of the fact that both receptors can be coupled to Gi/0 protein, the present results give support for the existence of antagonistic functional D4R-MOR receptor-receptor interactions in the adaptive changes occurring in MOR of striosomes on continuous administration of morphine. View Full-Text
Keywords: morphine; PD168,077; µ opioid receptor; dopamine D4 receptor; G proteins; caudate putamen; striosomes; addiction morphine; PD168,077; µ opioid receptor; dopamine D4 receptor; G proteins; caudate putamen; striosomes; addiction
MDPI and ACS Style

Suárez-Boomgaard, D.; Gago, B.; Valderrama-Carvajal, A.; Roales-Buján, R.; Van Craenenbroeck, K.; Duchou, J.; Borroto-Escuela, D.O.; Medina-Luque, J.; De la Calle, A.; Fuxe, K.; Rivera, A. Dopamine D4 Receptor Counteracts Morphine-Induced Changes in µ Opioid Receptor Signaling in the Striosomes of the Rat Caudate Putamen. Int. J. Mol. Sci. 2014, 15, 1481-1498.

AMA Style

Suárez-Boomgaard D, Gago B, Valderrama-Carvajal A, Roales-Buján R, Van Craenenbroeck K, Duchou J, Borroto-Escuela DO, Medina-Luque J, De la Calle A, Fuxe K, Rivera A. Dopamine D4 Receptor Counteracts Morphine-Induced Changes in µ Opioid Receptor Signaling in the Striosomes of the Rat Caudate Putamen. International Journal of Molecular Sciences. 2014; 15(1):1481-1498.

Chicago/Turabian Style

Suárez-Boomgaard, Diana; Gago, Belén; Valderrama-Carvajal, Alejandra; Roales-Buján, Ruth; Van Craenenbroeck, Kathleen; Duchou, Jolien; Borroto-Escuela, Dasiel O.; Medina-Luque, José; De la Calle, Adelaida; Fuxe, Kjell; Rivera, Alicia. 2014. "Dopamine D4 Receptor Counteracts Morphine-Induced Changes in µ Opioid Receptor Signaling in the Striosomes of the Rat Caudate Putamen" Int. J. Mol. Sci. 15, no. 1: 1481-1498.

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop