Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from Phaseolus vulgaris L.

Rodríguez, Lyanne; Montecino-Garrido, Héctor Leonardo; Lagos, Felipe; Carrasco, Basilio; Palomo, Iván; Ormazabal, Paulina; Trostchansky, Andrés; Fuentes, Eduardo

doi:10.3390/molecules31030488

Open AccessArticle

Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from Phaseolus vulgaris L.

by

Lyanne Rodríguez

^1,†

,

Héctor Leonardo Montecino-Garrido

^2,†

,

Felipe Lagos

¹

,

Basilio Carrasco

²,

Iván Palomo

¹

,

Paulina Ormazabal

³

,

Andrés Trostchansky

^4,*

and

Eduardo Fuentes

^1,*

¹

Thrombosis and Healthy Aging Research Center, VITALIS Longevity Center, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, Chile

²

Centro de Estudios en Alimentos Procesados-CEAP, Conicyt, Programa Regional R20F0001, Gore Maule, Talca 3460000, Chile

³

Escuela de Obstetricia, Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Lota 2465, Providencia, Santiago 8420524, Chile

⁴

Departamento de Bioquímica and Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República, Montevideo 11200, Uruguay

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Molecules 2026, 31(3), 488; https://doi.org/10.3390/molecules31030488

Submission received: 12 December 2025 / Revised: 23 January 2026 / Accepted: 27 January 2026 / Published: 30 January 2026

(This article belongs to the Special Issue Natural Bioactives and Functional Ingredients in Foods)

Download

Browse Figures

Review Reports Versions Notes

Abstract

Dietary bioactive compounds are increasingly explored as complementary cardioprotective strategies, and the nitration of unsaturated fatty acids has emerged as a process capable of enhancing antiplatelet properties. This study investigated whether Phaseolus vulgaris L. extracts can generate nitrated fatty acids under gastric-like conditions and evaluated their effects on human platelet function. Bean extracts and major fatty acids were nitrated in vitro and tested using washed platelets to assess cytotoxicity, TRAP-6 and collagen-induced aggregation, activation markers (P-selectin, CD63), and mitochondrial responses including membrane potential, ROS production, and Ca²⁺ dynamics. Nitrated extracts markedly inhibited TRAP-6 induced aggregation (IC₅₀ ≈ 1.8 mg/mL), whereas non-nitrated extracts showed minimal activity; this effect was reversed by β-mercaptoethanol, indicating dependence on electrophilic nitroalkenes. Fractionation revealed that the lipidic fraction accounted for most of the antiplatelet effect, and isolated nitrated fatty acids (NO₂-LN, NO₂-LA, NO₂-OA) displayed stronger inhibition than their native counterparts without increasing cytotoxicity. Nitrated species additionally reduced mitochondrial membrane potential and granule secretion without elevating ROS. These findings identify Phaseolus vulgaris L. as a natural source of bioactive nitrated fatty acids and support their potential as nutraceutical agents capable of modulating platelet activation and contributing to cardiovascular risk reduction.

Keywords: cardiovascular diseases; platelets; bean nitrated extracts; nitrated fatty acids

Share and Cite

MDPI and ACS Style

Rodríguez, L.; Montecino-Garrido, H.L.; Lagos, F.; Carrasco, B.; Palomo, I.; Ormazabal, P.; Trostchansky, A.; Fuentes, E. Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from Phaseolus vulgaris L. Molecules 2026, 31, 488. https://doi.org/10.3390/molecules31030488

AMA Style

Rodríguez L, Montecino-Garrido HL, Lagos F, Carrasco B, Palomo I, Ormazabal P, Trostchansky A, Fuentes E. Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from Phaseolus vulgaris L. Molecules. 2026; 31(3):488. https://doi.org/10.3390/molecules31030488

Chicago/Turabian Style

Rodríguez, Lyanne, Héctor Leonardo Montecino-Garrido, Felipe Lagos, Basilio Carrasco, Iván Palomo, Paulina Ormazabal, Andrés Trostchansky, and Eduardo Fuentes. 2026. "Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from Phaseolus vulgaris L." Molecules 31, no. 3: 488. https://doi.org/10.3390/molecules31030488

APA Style

Rodríguez, L., Montecino-Garrido, H. L., Lagos, F., Carrasco, B., Palomo, I., Ormazabal, P., Trostchansky, A., & Fuentes, E. (2026). Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from Phaseolus vulgaris L. Molecules, 31(3), 488. https://doi.org/10.3390/molecules31030488

Article Menu

Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from Phaseolus vulgaris L.

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI