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Correction: Yang et al. Synthesis and Fungicidal Activities of (Z/E)-3,7-Dimethyl-2,6-octadienamide and Its 6,7-Epoxy Analogues. Molecules 2015, 20, 21023–21036
 
 
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Correction published on 26 March 2026, see Molecules 2026, 31(7), 1081.
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Article

Omics Reveals the Antibacterial Mechanism of Dihydromyricetin and Vine Tea Extract Against Staphylococcus aureus via Cell Wall and Membrane Disruption

by
Qiaoni Hui
,
Ting Li
,
Keke He
,
Wei Ma
,
Ying Guo
,
Yao Zhang
and
Liya Song
*
Department of Cosmetics, School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China
*
Author to whom correspondence should be addressed.
Molecules 2026, 31(2), 313; https://doi.org/10.3390/molecules31020313
Submission received: 13 December 2025 / Revised: 11 January 2026 / Accepted: 13 January 2026 / Published: 16 January 2026 / Corrected: 26 March 2026
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Abstract

Staphylococcus aureus (S. aureus) is a common pathogen that threatens healthcare and food safety. Vine tea extract (VTE) and its major active component, dihydromyricetin (DMY), show antibacterial activity. However, their mechanisms of action are not fully understood. In this study, we combined proteomics and lipidomics, with RT–qPCR validation of selected differentially expressed genes, to investigate how DMY and VTE affect S. aureus. Proteomics identified 210 and 535 differentially expressed proteins (DEPs) in the DMY-treated and VTE-treated groups, respectively. These DEPs were mainly enriched in cell wall- and membrane-associated pathways. DMY markedly increased proteins involved in fatty acid degradation, glyceride metabolism, and cell wall synthesis. In contrast, VTE increased proteins related to heme/iron acquisition and cell wall degradation. In addition, VTE altered proteins involved in pyrimidine metabolism and aminoacyl-tRNA biosynthesis, suggesting that non-DMY components in VTE may contribute to the antibacterial activity through additional pathways. Lipidomics further indicated membrane lipid remodeling, including increased fatty acid unsaturation and shorter acyl chain length. Collectively, DMY and VTE may inhibit S. aureus growth by remodeling membrane lipids and disturbing cell wall–cell membrane homeostasis. These findings provide mechanistic support for further development of DMY and VTE as natural antimicrobial candidates.
Keywords: Staphylococcus aureus; dihydromyricetin; vine tea extract; antibacterial mechanism; proteomics; lipidomics Staphylococcus aureus; dihydromyricetin; vine tea extract; antibacterial mechanism; proteomics; lipidomics

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MDPI and ACS Style

Hui, Q.; Li, T.; He, K.; Ma, W.; Guo, Y.; Zhang, Y.; Song, L. Omics Reveals the Antibacterial Mechanism of Dihydromyricetin and Vine Tea Extract Against Staphylococcus aureus via Cell Wall and Membrane Disruption. Molecules 2026, 31, 313. https://doi.org/10.3390/molecules31020313

AMA Style

Hui Q, Li T, He K, Ma W, Guo Y, Zhang Y, Song L. Omics Reveals the Antibacterial Mechanism of Dihydromyricetin and Vine Tea Extract Against Staphylococcus aureus via Cell Wall and Membrane Disruption. Molecules. 2026; 31(2):313. https://doi.org/10.3390/molecules31020313

Chicago/Turabian Style

Hui, Qiaoni, Ting Li, Keke He, Wei Ma, Ying Guo, Yao Zhang, and Liya Song. 2026. "Omics Reveals the Antibacterial Mechanism of Dihydromyricetin and Vine Tea Extract Against Staphylococcus aureus via Cell Wall and Membrane Disruption" Molecules 31, no. 2: 313. https://doi.org/10.3390/molecules31020313

APA Style

Hui, Q., Li, T., He, K., Ma, W., Guo, Y., Zhang, Y., & Song, L. (2026). Omics Reveals the Antibacterial Mechanism of Dihydromyricetin and Vine Tea Extract Against Staphylococcus aureus via Cell Wall and Membrane Disruption. Molecules, 31(2), 313. https://doi.org/10.3390/molecules31020313

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