Next Article in Journal
Pomegranate Peel as a Sustainable Additive for Baijiu Fermentation: Physicochemical and Flavor Analysis with Process Optimization
Next Article in Special Issue
From Analytical Profiling to Liposomal Delivery: Cannabinol as a Model for Antioxidant Encapsulation and Diffusion Enhancement
Previous Article in Journal
Lithium Induces Oxidative Stress, Apoptotic Cell Death, and G2/M Phase Cell Cycle Arrest in A549 Lung Cancer Cells
Previous Article in Special Issue
The Pharmaceutical and Pharmacological Potential Applications of Bilosomes as Nanocarriers for Drug Delivery
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 30
Issue 8
10.3390/molecules30081798
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Structure Elucidation and Characterization of Novel Glycolipid Biosurfactant Produced by Rouxiella badensis DSM 100043T

by
Andre Fahriz Perdana Harahap
1,
Jürgen Conrad
2,
Mario Wolf
2,
Jens Pfannstiel
3,
Iris Klaiber
3,
Jakob Grether
1,
Eric Hiller
1,
Maliheh Vahidinasab
1,
Hanna Salminen
4,
Chantal Treinen
5,
Elvio Henrique Benatto Perino
1 and
Rudolf Hausmann
1,*
1
Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstr. 12, 70599 Stuttgart, Germany
2
Department of Organic Chemistry (130b), Institute of Chemistry, University of Hohenheim, Garbenstr. 30, 70599 Stuttgart, Germany
3
Mass Spectrometry Unit, Core Facility Hohenheim, University of Hohenheim, Ottilie-Zeller-Weg 2, 70599 Stuttgart, Germany
4
Department of Food Material Science (150g), Institute of Food Science and Biotechnology, University of Hohenheim, Garbenstr. 21/25, 70599 Stuttgart, Germany
5
Cellular Agriculture, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany
*
Author to whom correspondence should be addressed.
Molecules 2025, 30(8), 1798; https://doi.org/10.3390/molecules30081798
Submission received: 21 March 2025 / Revised: 14 April 2025 / Accepted: 14 April 2025 / Published: 17 April 2025
(This article belongs to the Special Issue Lipids and Surfactants in Delivery Systems)
Browse Figures
Versions Notes

Abstract

Microbial biosurfactants have become increasingly attractive as promising ingredients for environmentally friendly products. The reasons for this are their generally good performance and biodegradability, low toxicity, production from renewable raw materials, and benefits for the environment perceived by consumers. In this study, we investigated the chemical structure and properties of a novel glycolipid from a new biosurfactant-producing strain, Rouxiella badensis DSM 100043T. Bioreactor cultivation was performed at 30 °C and pH 7.0 for 28 h using 15 g/L glycerol as a carbon source. The glycolipid was successfully purified from the ethyl acetate extract of the supernatant using medium pressure liquid chromatography (MPLC). The structure of the glycolipid was determined by one- and two-dimensional (1H and 13C) nuclear magnetic resonance (NMR) and confirmed by liquid chromatography electrospray ionization mass spectrometry (LC-ESI/MS). NMR analysis revealed the hydrophilic moiety as a glucose molecule and the hydrophobic moieties as 3-hydroxy-5-dodecenoic acid and 3-hydroxydecanoic acid, which are linked with the glucose by ester bonds at the C2 and C3 positions. Surface tension measurement with tensiometry indicated that the glucose–lipid could reduce the surface tension of water from 72.05 mN/m to 24.59 mN/m at 25 °C with a very low critical micelle concentration (CMC) of 5.69 mg/L. Moreover, the glucose–lipid demonstrated very good stability in maintaining emulsification activity at pH 2–8, a temperature of up to 100 °C, and a NaCl concentration of up to 15%. These results show that R. badensis DSM 100043T produced a novel glycolipid biosurfactant with excellent surface-active properties, making it promising for further research or industrial applications.
Keywords: Rouxiella badensis; biosurfactants; emulsification; glucose–lipid; glycolipid; structure elucidation Rouxiella badensis; biosurfactants; emulsification; glucose–lipid; glycolipid; structure elucidation

Share and Cite

MDPI and ACS Style

Harahap, A.F.P.; Conrad, J.; Wolf, M.; Pfannstiel, J.; Klaiber, I.; Grether, J.; Hiller, E.; Vahidinasab, M.; Salminen, H.; Treinen, C.; et al. Structure Elucidation and Characterization of Novel Glycolipid Biosurfactant Produced by Rouxiella badensis DSM 100043T. Molecules 2025, 30, 1798. https://doi.org/10.3390/molecules30081798

AMA Style

Harahap AFP, Conrad J, Wolf M, Pfannstiel J, Klaiber I, Grether J, Hiller E, Vahidinasab M, Salminen H, Treinen C, et al. Structure Elucidation and Characterization of Novel Glycolipid Biosurfactant Produced by Rouxiella badensis DSM 100043T. Molecules. 2025; 30(8):1798. https://doi.org/10.3390/molecules30081798

Chicago/Turabian Style

Harahap, Andre Fahriz Perdana, Jürgen Conrad, Mario Wolf, Jens Pfannstiel, Iris Klaiber, Jakob Grether, Eric Hiller, Maliheh Vahidinasab, Hanna Salminen, Chantal Treinen, and et al. 2025. "Structure Elucidation and Characterization of Novel Glycolipid Biosurfactant Produced by Rouxiella badensis DSM 100043T" Molecules 30, no. 8: 1798. https://doi.org/10.3390/molecules30081798

APA Style

Harahap, A. F. P., Conrad, J., Wolf, M., Pfannstiel, J., Klaiber, I., Grether, J., Hiller, E., Vahidinasab, M., Salminen, H., Treinen, C., Perino, E. H. B., & Hausmann, R. (2025). Structure Elucidation and Characterization of Novel Glycolipid Biosurfactant Produced by Rouxiella badensis DSM 100043T. Molecules, 30(8), 1798. https://doi.org/10.3390/molecules30081798

Article Metrics

Back to TopTop