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10.3390/molecules28227496
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Article

Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products

by
Qian Han
1,2,†,
Ling Fan
3,†,
Xiuying Liu
1,2,*,
Yiwei Tang
4,
Pingping Wang
1,2,
Zaixi Shu
1,2,
Wei Zhang
1,2 and
Lijie Zhu
1,2
1
School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430028, China
2
Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Wuhan 430028, China
3
College of Food Science and Technology, Bohai University, Jinzhou 121013, China
4
College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2023, 28(22), 7496; https://doi.org/10.3390/molecules28227496
Submission received: 21 September 2023 / Revised: 6 November 2023 / Accepted: 7 November 2023 / Published: 9 November 2023
(This article belongs to the Section Materials Chemistry)
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Abstract

Quantum dot nanobeads (QBs) were used as signal source to develop competitive lateral flow immunoassay (LFIA) for the detection of chloramphenicol (CAP). The quantitative detection of CAP was achieved by calculating the total color difference (∆E) values of the test line (T line) using the images of test strips. QB-based LFIA (QBs-LFIA) allowed the effective dynamic linear detection of CAP in the range of 0.1–1.5 ng/mL. The limit of detection (LOD) was 3.0 ng/mL, which was 50 and 667 times lower than those achieved for two different brands of colloidal gold kits. The recoveries of CAP during real-sample detection were 82.82–104.91% at spiked levels of 0.1, 0.7, and 1.5 ng/mL. These results indicate that the developed QBs-LFIA facilitates the sensitive detection of CAP.
Keywords: chloramphenicol; lateral flow immunoassay; quantum dot nanobeads; fluorescent test strip chloramphenicol; lateral flow immunoassay; quantum dot nanobeads; fluorescent test strip

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MDPI and ACS Style

Han, Q.; Fan, L.; Liu, X.; Tang, Y.; Wang, P.; Shu, Z.; Zhang, W.; Zhu, L. Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products. Molecules 2023, 28, 7496. https://doi.org/10.3390/molecules28227496

AMA Style

Han Q, Fan L, Liu X, Tang Y, Wang P, Shu Z, Zhang W, Zhu L. Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products. Molecules. 2023; 28(22):7496. https://doi.org/10.3390/molecules28227496

Chicago/Turabian Style

Han, Qian, Ling Fan, Xiuying Liu, Yiwei Tang, Pingping Wang, Zaixi Shu, Wei Zhang, and Lijie Zhu. 2023. "Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products" Molecules 28, no. 22: 7496. https://doi.org/10.3390/molecules28227496

APA Style

Han, Q., Fan, L., Liu, X., Tang, Y., Wang, P., Shu, Z., Zhang, W., & Zhu, L. (2023). Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products. Molecules, 28(22), 7496. https://doi.org/10.3390/molecules28227496

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