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Volume 27
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10.3390/molecules27238262
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Review

Molecular Methods for Identification and Quantification of Foodborne Pathogens

by
Min Zhang
1,
Jiajia Wu
1,
Zhaoai Shi
1,
Aocheng Cao
1,2,
Wensheng Fang
1,2,
Dongdong Yan
1,2,
Qiuxia Wang
1,2 and
Yuan Li
1,2,*
1
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Beijing Innovation Consortium of Agriculture Research System, Beijing 100193, China
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(23), 8262; https://doi.org/10.3390/molecules27238262
Submission received: 24 October 2022 / Revised: 12 November 2022 / Accepted: 21 November 2022 / Published: 26 November 2022
(This article belongs to the Special Issue Featured Review Papers in Food Chemistry)
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Abstract

Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne illness. We summarize simple, rapid, specific, and highly effective molecular technology that is used to detect and identify foodborne pathogens, including polymerase chain reaction, isothermal amplification, loop-mediated isothermal amplification, nucleic acid sequence-based amplification, as well as gene chip and gene probe technology. The principles of their operation, the research supporting their application, and the advantages and disadvantages of each technology are summarized.
Keywords: polymerase chain reaction; isothermal amplification; loop-mediated isothermal amplification; nucleic acid sequence-based amplification; gene chip; gene probe technology polymerase chain reaction; isothermal amplification; loop-mediated isothermal amplification; nucleic acid sequence-based amplification; gene chip; gene probe technology

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MDPI and ACS Style

Zhang, M.; Wu, J.; Shi, Z.; Cao, A.; Fang, W.; Yan, D.; Wang, Q.; Li, Y. Molecular Methods for Identification and Quantification of Foodborne Pathogens. Molecules 2022, 27, 8262. https://doi.org/10.3390/molecules27238262

AMA Style

Zhang M, Wu J, Shi Z, Cao A, Fang W, Yan D, Wang Q, Li Y. Molecular Methods for Identification and Quantification of Foodborne Pathogens. Molecules. 2022; 27(23):8262. https://doi.org/10.3390/molecules27238262

Chicago/Turabian Style

Zhang, Min, Jiajia Wu, Zhaoai Shi, Aocheng Cao, Wensheng Fang, Dongdong Yan, Qiuxia Wang, and Yuan Li. 2022. "Molecular Methods for Identification and Quantification of Foodborne Pathogens" Molecules 27, no. 23: 8262. https://doi.org/10.3390/molecules27238262

APA Style

Zhang, M., Wu, J., Shi, Z., Cao, A., Fang, W., Yan, D., Wang, Q., & Li, Y. (2022). Molecular Methods for Identification and Quantification of Foodborne Pathogens. Molecules, 27(23), 8262. https://doi.org/10.3390/molecules27238262

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