Exogenous Sodium Nitroprusside Mitigates Salt Stress in Lentil (Lens culinaris Medik.) by Affecting the Growth, Yield, and Biochemical Properties
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Arrangements
2.2. NaCl and SNP Treatments
2.3. Determination of Growth and Yield Parameters
2.4. Estimation of Leaf Relative Water Content
2.5. Determination of Leaf Greenness Index
2.6. Estimation of H2O2 and MDA Content
2.7. Antioxidant Enzyme Assays
2.8. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Treatments | Shoot Length (cm) | Root Length (cm) | Leaf Relative Water Content (%) | Leaf Greenness Index |
---|---|---|---|---|
T1 = 0 mM of NaCl + 0 µM of SNP (Control) | 40.12 ± 1.18 a | 20.82 ± 0.62 a | 81.58 ± 1.88 a | 12.51 ± 0.97 ab |
T2 = 50 mM of NaCl + 0 µM of SNP | 32.18 ± 0.83 c | 12.55 ± 0.95 de | 64.48 ± 2.05 d | 9.42 ± 1.22 cd |
T3 = 100 mM of NaCl + 0 µM of SNP | 23.41 ± 1.24 e | 7.62 ± 0.76 f | 42.86 ± 1.06 e | 7.28 ± 1.05 d |
T4 = 50 mM of NaCl + 50 µM of SNP | 36.66 ± 1.14 b | 16.23 ± 0.96 bc | 74.27 ± 1.71 b | 12.27 ± 1.07 ab |
T5 = 100 mM of NaCl + 50 µM of SNP | 30.55 ± 0.85 c | 13.71 ± 0.83 cd | 69.11 ± 1.68 c | 10.24 ± 0.87 bc |
T6 = 50 mM of NaCl + 100 µM of SNP | 38.72 ± 1.02 ab | 18.42 ± 0.73 ab | 84.32 ± 2.46 a | 14.80 ± 0.97 a |
T7 = 100 mM of NaCl + 100 µM of SNP | 27.18 ± 0.82 d | 10.22 ± 0.87 ef | 65.33 ± 1.99 d | 11.71 ± 0.99 bc |
Treatments | Number of Branches Plant−1 | Number of Pods Plant−1 | Number of Seeds Plant−1 | Seed Yield (g Plant−1) | Biomass (g Plant−1) |
---|---|---|---|---|---|
T1 = 0 mM of NaCl + 0 µM of SNP | 10.50 ± 0.53 b | 36.52 ± 2.50 b | 54.50 ± 3.18 b | 1.32 ± 0.18 ab | 9.17 ± 0.73 b |
T2 = 50 mM of NaCl + 0 µM of SNP | 7.25 ± 0.80 c | 27.51 ± 2.11 d | 34.52 ± 1.97 e | 0.86 ± 0.04 ab | 7.82 ± 0.58 b |
T3 = 100 mM of NaCl + 0 µM of SNP | 4.91 ± 0.69 d | 20.04 ± 1.73 e | 21.54 ± 1.06 f | 0.53 ± 0.06 b | 4.22 ± 0.71 c |
T4 = 50 mM of NaCl + 50 µM of SNP | 8.66 ± 0.82 c | 34.54 ± 1.52 bc | 49.48 ± 2.71 c | 1.23 ± 0.08 ab | 8.95 ± 0.84 b |
T5 = 100 mM of NaCl + 50 µM of SNP | 7.33 ± 0.35 c | 32.20 ± 2.09 c | 39.50 ± 2.44 d | 0.93 ± 0.12 ab | 5.42 ± 0.62 c |
T6 = 50 mM of NaCl + 100 µM of SNP | 14.58 ± 0.72 a | 48.75 ± 2.81 a | 66.25 ± 2.70 a | 1.65 ± 0.21 a | 14.15 ± 1.06 a |
T7 = 100 mM of NaCl + 100 µM of SNP | 8.16 ± 1.01 c | 33.75 ± 2.13 bc | 48.52 ± 2.32 c | 1.21 ± 0.13 ab | 8.38 ± 0.74 b |
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Yasir, T.A.; Khan, A.; Skalicky, M.; Wasaya, A.; Rehmani, M.I.A.; Sarwar, N.; Mubeen, K.; Aziz, M.; Hassan, M.M.; Hassan, F.A.S.; et al. Exogenous Sodium Nitroprusside Mitigates Salt Stress in Lentil (Lens culinaris Medik.) by Affecting the Growth, Yield, and Biochemical Properties. Molecules 2021, 26, 2576. https://doi.org/10.3390/molecules26092576
Yasir TA, Khan A, Skalicky M, Wasaya A, Rehmani MIA, Sarwar N, Mubeen K, Aziz M, Hassan MM, Hassan FAS, et al. Exogenous Sodium Nitroprusside Mitigates Salt Stress in Lentil (Lens culinaris Medik.) by Affecting the Growth, Yield, and Biochemical Properties. Molecules. 2021; 26(9):2576. https://doi.org/10.3390/molecules26092576
Chicago/Turabian StyleYasir, Tauqeer Ahmad, Ayesha Khan, Milan Skalicky, Allah Wasaya, Muhammad Ishaq Asif Rehmani, Naeem Sarwar, Khuram Mubeen, Mudassir Aziz, Mohamed M. Hassan, Fahmy A. S. Hassan, and et al. 2021. "Exogenous Sodium Nitroprusside Mitigates Salt Stress in Lentil (Lens culinaris Medik.) by Affecting the Growth, Yield, and Biochemical Properties" Molecules 26, no. 9: 2576. https://doi.org/10.3390/molecules26092576