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Active Substances from Callicarpa nudiflora Exert Anti-Cervicitis Effects and Regulate NLRP3-Associated Inflammation

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State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau 999078, China
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Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Domenico Trombetta
Molecules 2021, 26(20), 6217; https://doi.org/10.3390/molecules26206217
Received: 1 September 2021 / Revised: 1 October 2021 / Accepted: 11 October 2021 / Published: 14 October 2021
Luohuazizhu suppository is a Traditional Chinese Medicine used in clinic to treat cervicitis, which is prepared from Callicarpa nudiflora Hook. et Arn (C. nudiflora), an herbal Chinese medicine named Luohuazizhu. This study aimed to figure out the active constituents of C. nudiflora and the potential mechanism for its anti-cervicitis effect. The ethanol extract in C. nudiflora (CNE) and the different fractions of CNE extracted by petroleum ether (CNE-p), dichloromethane (CNE-d), and n-butanol (CNE-b) were tested in vivo for their anti-cervicitis effects. Then the isolated compounds from the CNE-p were tested in vitro for their anti-inflammatory activities. The results displayed that CNE-p, CNE-d, and CNE-b exhibited adequate anti-cervicitis effects, with CNE-p showing the highest efficacy. Further experiment demonstrated that CNE-p could significantly inhibit the expression of NLRP3 in vitro. Six diterpenoids obtained from the CNE-p showed the ability to regulate inflammatory factor levels in vitro. Among these compounds, compounds 1 (callicarpic acid A) and 2 (syn-3,4-seco-12S-hydroxy-15,16-epoxy-4(18),8(17),3(16),14(15)-labdatetraen-3-oic acid) were the most effective agents, and they also inhibited the expression level of NLRP3 in vitro. The results confirmed that C. nudiflora has significant anti-cervicitis effects and the diterpenoids were most likely to be its active components. These data provide scientific support for the clinic usage of Luohuazizhu suppository and the development of new agents in treating cervicitis. View Full-Text
Keywords: Callicarpa nudiflora; active substances; anti-cervicitis; diterpenoids; NLRP3 Callicarpa nudiflora; active substances; anti-cervicitis; diterpenoids; NLRP3
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MDPI and ACS Style

Liu, T.; Wang, R.; Liu, C.; Lu, J.; Wang, Y.; Dong, L.; Zhang, X. Active Substances from Callicarpa nudiflora Exert Anti-Cervicitis Effects and Regulate NLRP3-Associated Inflammation. Molecules 2021, 26, 6217. https://doi.org/10.3390/molecules26206217

AMA Style

Liu T, Wang R, Liu C, Lu J, Wang Y, Dong L, Zhang X. Active Substances from Callicarpa nudiflora Exert Anti-Cervicitis Effects and Regulate NLRP3-Associated Inflammation. Molecules. 2021; 26(20):6217. https://doi.org/10.3390/molecules26206217

Chicago/Turabian Style

Liu, Tianchi, Ruiqi Wang, Chenpeng Liu, Jiahong Lu, Yitao Wang, Lin Dong, and Xiaopo Zhang. 2021. "Active Substances from Callicarpa nudiflora Exert Anti-Cervicitis Effects and Regulate NLRP3-Associated Inflammation" Molecules 26, no. 20: 6217. https://doi.org/10.3390/molecules26206217

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