The Novel Role of MIF in the Secretion of IL-25, IL-31, and IL-33 from PBMC of Patients with Rheumatoid Arthritis
Abstract
:1. Introduction
2. Results
2.1. Demographic and Clinical Characteristics of Donors of PBMC
2.2. Effect of LPS and rhMIF on IL-25, IL-31, and IL-33 Secretion from PBMC of CS and RA Patients
2.3. Effect of Interaction between LPS Plus rhMIF on IL-25, IL-31, and IL-33 Secretion from PBMC of CS and RA Patients
2.4. Analysis of Interactions between Groups of Study or Treatments with Stimuli Effects
3. Discussion
4. Materials and Methods
4.1. Participants
4.2. Isolation of PBMC and Culture Protocol
4.3. Cytokine Assays
4.4. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Investigated Parameters | Sum of Squares (SS) | Mean of Squares (MS) | F | p Value | Sum of Squares (SS) | Mean of Squares (MS) | F | p Value |
---|---|---|---|---|---|---|---|---|
CS | RA | |||||||
IL-25 | ||||||||
rhMIF | 460 | 460 | 2.163 | 0.151 | 830.2 | 830.2 | 19.025 | <0.001 |
LPS | 5440.8 | 5440.8 | 25.578 | <0.001 | 370.7 | 370.7 | 8.495 | 0.006 |
rhMIF + LPS | 117.9 | 117.9 | 0.554 | 0.462 | 17.8 | 17.8 | 0.407 | 0.528 |
Error | 6806.9 | 212.7 | 1396.4 | 43.637 | ||||
Total SS | 21,988.9 | 5565.4 | ||||||
IL-31 | ||||||||
rhMIF | 8690.6 | 8690.6 | 4.2 | 0.049 | 16,992 | 16,992 | 32.472 | <0.001 |
LPS | 96,602.9 | 96,602.9 | 46.692 | <0.001 | 14,176 | 14,176 | 27.091 | <0.001 |
rhMIF + LPS | 905.8 | 905.8 | 0.438 | 0.513 | 208.3 | 208.3 | 0.398 | 0.533 |
Error | 66,206.7 | 2069 | 16,745 | 523.3 | ||||
Total SS | 380,120 | 147,769 | ||||||
IL-33 | ||||||||
rhMIF | 40.6 | 40.6 | 2.672 | 0.112 | 156.2 | 156.2 | 16.427 | <0.001 |
LPS | 402.3 | 402.3 | 26.453 | <0.001 | 122.6 | 122.6 | 12.898 | 0.001 |
rhMIF + LPS | 4.5 | 4.5 | 0.295 | 0.591 | 0.14 | 0.14 | 0.015 | 0.903 |
Error | 486.7 | 15.209 | 304.2 | 9.5 | ||||
Total SS | 2266.1 | 1530.8 |
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García-Arellano, S.; Hernández-Palma, L.A.; Cerpa-Cruz, S.; Sánchez-Zuno, G.A.; Herrera-Godina, M.G.; Muñoz-Valle, J.F. The Novel Role of MIF in the Secretion of IL-25, IL-31, and IL-33 from PBMC of Patients with Rheumatoid Arthritis. Molecules 2021, 26, 4968. https://doi.org/10.3390/molecules26164968
García-Arellano S, Hernández-Palma LA, Cerpa-Cruz S, Sánchez-Zuno GA, Herrera-Godina MG, Muñoz-Valle JF. The Novel Role of MIF in the Secretion of IL-25, IL-31, and IL-33 from PBMC of Patients with Rheumatoid Arthritis. Molecules. 2021; 26(16):4968. https://doi.org/10.3390/molecules26164968
Chicago/Turabian StyleGarcía-Arellano, Samuel, Luis Alexis Hernández-Palma, Sergio Cerpa-Cruz, Gabriela Athziri Sánchez-Zuno, Melva Guadalupe Herrera-Godina, and José Francisco Muñoz-Valle. 2021. "The Novel Role of MIF in the Secretion of IL-25, IL-31, and IL-33 from PBMC of Patients with Rheumatoid Arthritis" Molecules 26, no. 16: 4968. https://doi.org/10.3390/molecules26164968