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Open AccessArticle

Proteomic Characterisation of Lupin (Lupinus angustifolius) Milk as Influenced by Extraction Techniques, Seed Coat and Cultivars

1
College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia
2
Department of Food Science and Biotechnology, Faculty of Agriculture, University of Baghdad, Baghdad 10071, Iraq
3
Australia China Centre for Wheat Improvement (ACCWI), College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia
*
Authors to whom correspondence should be addressed.
The authors contributed equally to this work.
Molecules 2020, 25(8), 1782; https://doi.org/10.3390/molecules25081782
Received: 20 March 2020 / Revised: 6 April 2020 / Accepted: 10 April 2020 / Published: 13 April 2020
(This article belongs to the Section Analytical Chemistry)
Lupin seeds are rich in proteins and other essential ingredients that can help to improve human health. The protein contents in both whole and split seeds of two lupin cultivars (Mandleup and PBA Jurien) were used to produce the lupin milk using the cheesecloth and centrifuge method. Proteins were extracted from the lupin milk using thiourea/urea solubilization. The proteins were separated by a two-dimensional polyacrylamide gel electrophoresis and then identified with mass spectrometry. A total of 230 protein spots were identified, 60 of which showed differential abundances. The cheesecloth separation showed protein extractability much better than that of the centrifuge method for both the cultivars. The results from this study could offer guidance for future comparative analysis and identification of lupin milk protein and provide effective separation technique to determine specific proteins in the cheese-making process. View Full-Text
Keywords: Lupin; PBA Jurien; Mandelup; protein; two-dimensional gel electrophoresis; cheesecloth separation; centrifuge separation Lupin; PBA Jurien; Mandelup; protein; two-dimensional gel electrophoresis; cheesecloth separation; centrifuge separation
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MDPI and ACS Style

Al-Saedi, N.; Agarwal, M.; Ma, W.; Islam, S.; Ren, Y. Proteomic Characterisation of Lupin (Lupinus angustifolius) Milk as Influenced by Extraction Techniques, Seed Coat and Cultivars. Molecules 2020, 25, 1782.

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