Antiproliferative and Antimetastatic Effects of Praeruptorin C on Human Non–Small Cell Lung Cancer through Inactivating ERK/CTSD Signalling Pathways

Liu, Chien-Ming; Shen, Huan-Ting; Lin, Yi-An; Yu, Yung-Luen; Chen, Yong-Syuan; Liu, Chung-Jung; Hsieh, Yi-Hsien

doi:10.3390/molecules25071625

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

Antiproliferative and Antimetastatic Effects of Praeruptorin C on Human Non–Small Cell Lung Cancer through Inactivating ERK/CTSD Signalling Pathways

^1,2,

³,

^4,5,6,

²,

^7,8,*

and

Yi-Hsien Hsieh

^9,10,11,*

Department of Pulmonary Medicine, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung 40427, Taiwan

Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical, Taichung 40201, Taiwan

Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan

⁴

Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan

⁵

Center for Molecular Medicine, China Medical University Hospital, Taichung 40402, Taiwan

⁶

Department of Biotechnology, Asia University, Taichung 40402, Taiwan

⁷

Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

⁸

Regenetative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

⁹

Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan

¹⁰

Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan

¹¹

Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 40201, Taiwan

Show full affiliation list

Hide full affiliation list

Authors to whom correspondence should be addressed.

Molecules 2020, 25(7), 1625; https://doi.org/10.3390/molecules25071625

Received: 1 March 2020 / Revised: 26 March 2020 / Accepted: 31 March 2020 / Published: 1 April 2020

(This article belongs to the Section Medicinal Chemistry)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Praeruptorin C (PC) reportedly has beneficial effects in terms of antiinflammation, antihypertension, and antiplatelet aggregation, and it potentially has anticancer activity. However, the effect of PC on human non–small cell lung cancer (NSCLC) is largely unknown. Compared with the effects of praeruptorin A and praeruptorin B, we observed that PC significantly suppressed cell proliferation, colony formation, wound closure, and migration and invasion of NSCLC cells. It induced cell cycle arrest in the G0/G1 phase, downregulated cyclin D1 protein, and upregulated p21 protein. PC also significantly reduced the expression of cathepsin D (CTSD). In addition, the phosphorylation/activation of the ERK1/2 signalling pathway was significantly suppressed in PC-treated NSCLC cells. Cotreatment with PC and U0126 synergistically inhibited CTSD expression, cell migration, and cell invasion, which suggests that the ERK1/2 signalling pathway is involved in the downregulation of CTSD expression and invasion activity of NSCLC cells by PC. These findings are the first to demonstrate the inhibitory effects of PC in NSCLC progression. Therefore, PC may represent a novel strategy for treating NSCLC. View Full-Text

Keywords: Praeruptorin C; non-small cell lung cancer; proliferation; invasion; cathepsin D

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Share and Cite

MDPI and ACS Style

Liu, C.-M.; Shen, H.-T.; Lin, Y.-A.; Yu, Y.-L.; Chen, Y.-S.; Liu, C.-J.; Hsieh, Y.-H. Antiproliferative and Antimetastatic Effects of Praeruptorin C on Human Non–Small Cell Lung Cancer through Inactivating ERK/CTSD Signalling Pathways. Molecules 2020, 25, 1625. https://doi.org/10.3390/molecules25071625

AMA Style

Liu C-M, Shen H-T, Lin Y-A, Yu Y-L, Chen Y-S, Liu C-J, Hsieh Y-H. Antiproliferative and Antimetastatic Effects of Praeruptorin C on Human Non–Small Cell Lung Cancer through Inactivating ERK/CTSD Signalling Pathways. Molecules. 2020; 25(7):1625. https://doi.org/10.3390/molecules25071625

Chicago/Turabian Style

Liu, Chien-Ming, Huan-Ting Shen, Yi-An Lin, Yung-Luen Yu, Yong-Syuan Chen, Chung-Jung Liu, and Yi-Hsien Hsieh. 2020. "Antiproliferative and Antimetastatic Effects of Praeruptorin C on Human Non–Small Cell Lung Cancer through Inactivating ERK/CTSD Signalling Pathways" Molecules 25, no. 7: 1625. https://doi.org/10.3390/molecules25071625

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

Antiproliferative and Antimetastatic Effects of Praeruptorin C on Human Non–Small Cell Lung Cancer through Inactivating ERK/CTSD Signalling Pathways

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI