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Article

Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride

1
Department of Toxicology, Poznan University of Medical Sciences, 60-631 Poznań, Poland
2
Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland
3
Department of Fruit, Vegetable and Cereal Technology, Environmental and Life Science University, 51-630 Wrocław, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Halina Ekiert and Agnieszka Szopa
Molecules 2020, 25(6), 1268; https://doi.org/10.3390/molecules25061268
Received: 18 February 2020 / Revised: 6 March 2020 / Accepted: 9 March 2020 / Published: 11 March 2020
(This article belongs to the Special Issue Biological Activities of Natural Products)
The aim of this study was to compare the protective effects of chokeberry juice and silymarin against chemical-induced liver fibrosis in rats. Liver fibrosis was induced by CCl4 administered two days a week for six weeks. Two groups of rats were co-treated with chokeberry juice, 10 mL/kg/day. or silymarin as a positive control, 100 mg/kg/day for six weeks. Hepatic lipid peroxidation was suppressed by 50% and the activity of hepatic antioxidant enzymes was increased by 19%–173% in rats co-treated with CCl4 and substances tested as compared to rats administered CCl4 alone. Hepatic hydroxyproline was decreased by 24% only in rats treated with silymarin. The messenger RNA (mRNA) expression levels of fibrosis-related molecules, procollagen I, α-SMA, TIMP-1, TGFβ, and TNFα, which were significantly increased in the liver of CCl4-treated rats, were not modulated by substances tested. Histological evaluation revealed a slight protective effect of silymarin against fibrosis. However, in CCl4 + chokeberry-treated rats, the density of vacuolated hepatocytes was significantly lower than that in silymarin administered animals. Chokeberry juice did not demonstrate an antifibrotic effect in the applied experimental model of fibrosis, and the effect of the known antifibrotic agent, silymarin, was very limited. View Full-Text
Keywords: chokeberry; liver fibrosis; oxidative stress; silymarin chokeberry; liver fibrosis; oxidative stress; silymarin
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MDPI and ACS Style

Piotrowska-Kempisty, H.; Nowicki, M.; Jodynis-Liebert, J.; Kurpik, M.; Ewertowska, M.; Adamska, T.; Oszmiański, J.; Kujawska, M. Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride. Molecules 2020, 25, 1268. https://doi.org/10.3390/molecules25061268

AMA Style

Piotrowska-Kempisty H, Nowicki M, Jodynis-Liebert J, Kurpik M, Ewertowska M, Adamska T, Oszmiański J, Kujawska M. Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride. Molecules. 2020; 25(6):1268. https://doi.org/10.3390/molecules25061268

Chicago/Turabian Style

Piotrowska-Kempisty, Hanna, Michał Nowicki, Jadwiga Jodynis-Liebert, Monika Kurpik, Małgorzata Ewertowska, Teresa Adamska, Jan Oszmiański, and Małgorzata Kujawska. 2020. "Assessment of Hepatoprotective Effect of Chokeberry Juice in Rats Treated Chronically with Carbon Tetrachloride" Molecules 25, no. 6: 1268. https://doi.org/10.3390/molecules25061268

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