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10.3390/molecules25040791
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Article

Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer

by
Hyung Min Kim
1,
Yanxue Song
1,
Gyu Hwan Hyun
1,
Nguyen Phuoc Long
1,
Jeong Hill Park
1,
Yves S.Y. Hsieh
2 and
Sung Won Kwon
1,3,*
1
College of Pharmacy, Seoul National University, Seoul 08826, Korea
2
Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, Royal Institute of Technology (KTH), SE-106 91 Stockholm, Sweden
3
Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea
*
Author to whom correspondence should be addressed.
Molecules 2020, 25(4), 791; https://doi.org/10.3390/molecules25040791
Submission received: 12 December 2019 / Revised: 7 February 2020 / Accepted: 10 February 2020 / Published: 12 February 2020
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products 2019)
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Abstract

Panax ginseng (P. ginseng) is the most widely consumed herbal plant in Asia and is well-known for its various pharmacological properties. Many studies have been devoted to this natural product. However, polysaccharide’s components of ginseng and their biological effects have not been widely studied. In this study, white ginseng neutral polysaccharide (WGNP) and white ginseng acidic polysaccharide (WGAP) fractions were purified from P. ginseng roots. The chemical properties of WGNP and WGAP were investigated using various chromatography and spectroscopy techniques, including high-performance gel permeation chromatography, Fourier-transform infrared spectroscopy, and high-performance liquid chromatography with an ultra-violet detector. The antioxidant, anti-radical, and hydrogen peroxide scavenging activities were evaluated in vitro and in vivo using Caenorhabditis elegans as the model organism. Our in vitro data by ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), reducing power, ferrous ion chelating, and hydroxyl radical scavenging activity suggested that the WGAP with significantly higher uronic acid content and higher molecular weight exhibits a much stronger antioxidant effect as compared to that of WGNP. Similar antioxidant activity of WGAP was also confirmed in vivo by evaluating internal reactive oxygen species (ROS) concentration and lipid peroxidation. In conclusion, WGAP may be used as a natural antioxidant with potent scavenging and metal chelation properties.
Keywords: Panax ginseng; acidic polysaccharide; neutral polysaccharide; Caenorhabditis elegans; antioxidant effect Panax ginseng; acidic polysaccharide; neutral polysaccharide; Caenorhabditis elegans; antioxidant effect
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MDPI and ACS Style

Kim, H.M.; Song, Y.; Hyun, G.H.; Long, N.P.; Park, J.H.; Hsieh, Y.S.Y.; Kwon, S.W. Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer. Molecules 2020, 25, 791. https://doi.org/10.3390/molecules25040791

AMA Style

Kim HM, Song Y, Hyun GH, Long NP, Park JH, Hsieh YSY, Kwon SW. Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer. Molecules. 2020; 25(4):791. https://doi.org/10.3390/molecules25040791

Chicago/Turabian Style

Kim, Hyung Min, Yanxue Song, Gyu Hwan Hyun, Nguyen Phuoc Long, Jeong Hill Park, Yves S.Y. Hsieh, and Sung Won Kwon. 2020. "Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer" Molecules 25, no. 4: 791. https://doi.org/10.3390/molecules25040791

APA Style

Kim, H. M., Song, Y., Hyun, G. H., Long, N. P., Park, J. H., Hsieh, Y. S. Y., & Kwon, S. W. (2020). Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer. Molecules, 25(4), 791. https://doi.org/10.3390/molecules25040791

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