SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay

Putz, Ana-Maria; Ianăși, Cătălin; Dudás, Zoltán; Coricovac, Dorina; Watz, Claudia (Farcas); Len, Adél; Almásy, László; Sacarescu, Liviu; Dehelean, Cristina

doi:10.3390/molecules25030653

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

SiO₂-PVA-Fe(acac)₃ Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay

¹,

^2,*

Claudia (Farcas) Watz

^4,5,

²,

⁶ and

”Coriolan Dragulescu” Institute of Chemistry, Romanian Academy, Mihai Viteazul Bd., No. 24, 300223 Timişoara, Romania

Wigner Research Centre for Physics, POB 49 1525 Budapest, Hungary

Pharmacy II Department, Faculty of Pharmacy, “Victor Babes ̧” University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania

⁴

Centre for Energy Research, Konkoly-Thege 29-33, 1121 Budapest, Hungary

⁵

University of Pécs, Faculty of Engineering and Information technology, Boszorkány St. 2, 7624 Pécs, Hungary

⁶

Institute of Macromolecular Chemistry “Petru Poni”, Aleea Grigore Ghica Voda, nr. 41A 700487 Iasi, Romania

Author to whom correspondence should be addressed.

Academic Editor: Lorenzo Malavasi

Molecules 2020, 25(3), 653; https://doi.org/10.3390/molecules25030653

Received: 13 December 2019 / Revised: 31 January 2020 / Accepted: 1 February 2020 / Published: 4 February 2020

(This article belongs to the Special Issue Current Advances in Hybrid Functional Materials)

View Full-Text Download PDF

Browse Figures

Review Reports Citation Export

Abstract

A facile sol-gel route has been applied to synthesize hybrid silica-PVA-iron oxide nanocomposite materials. A step-by-step calcination (processing temperatures up to 400 °C) was applied in order to oxidize the organics together with the iron precursor. Transmission electron microscopy, X-ray diffraction, small angle neutron scattering, and nitrogen porosimetry were used to determine the temperature-induced morpho-textural modifications. In vitro cytotoxicity assay was conducted by monitoring the cell viability by the means of MTT assay to qualify the materials as MRI contrast agents or as drug carriers. Two cell lines were considered: the HaCaT (human keratinocyte cell line) and the A375 tumour cell line of human melanoma. Five concentrations of 10 µg/mL, 30 µg/mL, 50 µg/mL, 100 µg/mL, and 200 µg/mL were tested, while using DMSO (dimethylsulfoxid) and PBS (phosphate saline buffer) as solvents. The HaCaT and A375 cell lines were exposed to the prepared agent suspensions for 24 h. In the case of DMSO (dimethyl sulfoxide) suspensions, the effect on human keratinocytes migration and proliferation were also evaluated. The results indicate that only the concentrations of 100 μg/mL and 200 μg/mL of the nanocomposite in DMSO induced a slight decrease in the HaCaT cell viability. The PBS based in vitro assay showed that the nanocomposite did not present toxicity on the HaCaT cells, even at high doses (200 μg/mL agent). View Full-Text

Keywords: magnetic silica hybrids; maghemite; morpho-textural characterization; toxicology; sol-gel technique

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Share and Cite

MDPI and ACS Style

Putz, A.-M.; Ianăși, C.; Dudás, Z.; Coricovac, D.; Watz, C.; Len, A.; Almásy, L.; Sacarescu, L.; Dehelean, C. SiO₂-PVA-Fe(acac)₃ Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay. Molecules 2020, 25, 653. https://doi.org/10.3390/molecules25030653

AMA Style

Putz A-M, Ianăși C, Dudás Z, Coricovac D, Watz C, Len A, Almásy L, Sacarescu L, Dehelean C. SiO₂-PVA-Fe(acac)₃ Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay. Molecules. 2020; 25(3):653. https://doi.org/10.3390/molecules25030653

Chicago/Turabian Style

Putz, Ana-Maria, Cătălin Ianăși, Zoltán Dudás, Dorina Coricovac, Claudia Watz, Adél Len, László Almásy, Liviu Sacarescu, and Cristina Dehelean. 2020. "SiO₂-PVA-Fe(acac)₃ Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay" Molecules 25, no. 3: 653. https://doi.org/10.3390/molecules25030653

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

SiO₂-PVA-Fe(acac)₃ Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI