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Open AccessArticle

Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of Endophytic Fusarium culmorum Strains with Different Effects on Cereals

1
Department of Industrial and Environmental Microbiology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland
2
Department of Genetic and Microbiology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland
3
Department of Biochemistry and Biotechnology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland
4
Military Institute of Hygiene and Epidemiology, Lubelska St. 2, 24-100 Puławy, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Francisco Leon
Molecules 2020, 25(3), 616; https://doi.org/10.3390/molecules25030616
Received: 19 December 2019 / Revised: 24 January 2020 / Accepted: 28 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue Natural Products from Fungi)
Exopolymeric substances (EPS) can determine plant-microorganism interactions and have great potential as bioactive compounds. The different amounts of EPS obtained from cultures of three endophytic Fusarium culmorum strains with different aggressiveness—growth promoting (PGPF), deleterious (DRMO), and pathogenic towards cereal plants—depended on growth conditions. The EPS concentrations (under optimized culture conditions) were the lowest (0.2 g/L) in the PGPF, about three times higher in the DRMO, and five times higher in the pathogen culture. The EPS of these strains differed in the content of proteins, phenolic components, total sugars, glycosidic linkages, and sugar composition (glucose, mannose, galactose, and smaller quantities of arabinose, galactosamine, and glucosamine). The pathogen EPS exhibited the highest total sugar and mannose concentration. FTIR analysis confirmed the β configuration of the sugars. The EPS differed in the number and weight of polysaccharidic subfractions. The EPS of PGPF and DRMO had two subfractions and the pathogen EPS exhibited a subfraction with the lowest weight (5 kDa). The three EPS preparations (ethanol-precipitated EP, crude C, and proteolysed P) had antioxidant activity (particularly high for the EP-EPS soluble in high concentrations). The EP-EPS of the PGPF strain had the highest antioxidant activity, most likely associated with the highest content of phenolic compounds in this EPS. View Full-Text
Keywords: antioxidant activity; exopolymeric substances; Fusarium culmorum; sugar monomers; culture optimization; FTIR analyses antioxidant activity; exopolymeric substances; Fusarium culmorum; sugar monomers; culture optimization; FTIR analyses
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MDPI and ACS Style

Jaroszuk-Ściseł, J.; Nowak, A.; Komaniecka, I.; Choma, A.; Jarosz-Wilkołazka, A.; Osińska-Jaroszuk, M.; Tyśkiewicz, R.; Wiater, A.; Rogalski, J. Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of Endophytic Fusarium culmorum Strains with Different Effects on Cereals. Molecules 2020, 25, 616. https://doi.org/10.3390/molecules25030616

AMA Style

Jaroszuk-Ściseł J, Nowak A, Komaniecka I, Choma A, Jarosz-Wilkołazka A, Osińska-Jaroszuk M, Tyśkiewicz R, Wiater A, Rogalski J. Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of Endophytic Fusarium culmorum Strains with Different Effects on Cereals. Molecules. 2020; 25(3):616. https://doi.org/10.3390/molecules25030616

Chicago/Turabian Style

Jaroszuk-Ściseł, Jolanta; Nowak, Artur; Komaniecka, Iwona; Choma, Adam; Jarosz-Wilkołazka, Anna; Osińska-Jaroszuk, Monika; Tyśkiewicz, Renata; Wiater, Adrian; Rogalski, Jerzy. 2020. "Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of Endophytic Fusarium culmorum Strains with Different Effects on Cereals" Molecules 25, no. 3: 616. https://doi.org/10.3390/molecules25030616

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