Next Article in Journal
Cobalt Catalyzed C-P Bond Formation by Cross-Coupling of Boronic Acids with P(O)H Compounds in Presence of Zinc
Previous Article in Journal
Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics
Previous Article in Special Issue
Bioactive Plant Compounds in Coffee Charcoal (Coffeae carbo) Extract Inhibit Cytokine Release from Activated Human THP-1 Macrophages
Open AccessArticle

Phytochemical Characterization and In Vitro Anti-Inflammatory, Antioxidant and Antimicrobial Activity of Combretum Collinum Fresen Leaves Extracts from Benin

1
Institute of Pharmacy, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany
2
Institute for Medical Physics and Biophysics, Leipzig University, Härtelstr 16-18, 04107 Leipzig, Germany
3
Antimicrobial Agents Unit, Fraunhofer Institute for Cell Therapy and Immunology, Perlickstraße 1, 04103 Leipzig, Germany
4
Institute of Analytical Chemistry, Leipzig University, Linnéstr 3, 04103 Leipzig, Germany
5
Inter-Regional University of Industrial Engineering Biotechnologies and Applied Sciences, IRGIB Africa University, Cotonou 07 BP 231, Benin
6
Faculty of Natural and Environmental Sciences, Zittau/Görlitz University of Applied Sciences, 02763 Zittau, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Molecules 2020, 25(2), 288; https://doi.org/10.3390/molecules25020288
Received: 4 December 2019 / Revised: 30 December 2019 / Accepted: 7 January 2020 / Published: 10 January 2020
(This article belongs to the Special Issue Biological Activities of Medicinal Plants)
Leaves from Combretum collinum Fresen (Combretaceae) are commonly used for the treatment of inflammatory conditions, wound healing and bacterial infections in traditional West African medicine. This research focuses on the characterization of the phenolic profile and lipophilic compounds of leaves extracts of C. collinum. Studies of the in vitro anti-inflammatory activity were performed in TNFα stimulated HaCaT cells and antibacterial activity was evaluated with agar well diffusion and microdilution assays. Antioxidant activity was determined by DPPH and ABTS assays and compared to standards. The phytochemical studies confirmed myricetin-3-O-rhamnoside and myricetin-3-O-glucoside as major components of the leaves extracts, each contributing significantly to the antioxidant activity of the hydrophilic extracts. GC-MS analysis identified 19 substances that were confirmed by comparison with spectral library data and authentic standards. Combretum collinum aqueous leaves extract decreased pro-inflammatory mediators in TNFα stimulated HaCaT cells. Further investigations showed that myricetin-3-O-rhamnoside has an anti-inflammatory effect on IL-8 secretion. In the antimicrobial screening, the largest inhibition zones were found against S. epidermidis, MRSA and S. aureus. MIC values resulted in 275.0 µg/mL for S. epidermidis and 385.5 µg/mL for MRSA. The in vitro anti-inflammatory, antibacterial and antioxidant activity supports topical use of C. collinum leaves extracts in traditional West African medicine. View Full-Text
Keywords: Combretum collinum; anti-inflammatory; antimicrobial; keratinocytes; HaCaT Combretum collinum; anti-inflammatory; antimicrobial; keratinocytes; HaCaT
Show Figures

Graphical abstract

MDPI and ACS Style

Marquardt, P.; Seide, R.; Vissiennon, C.; Schubert, A.; Birkemeyer, C.; Ahyi, V.; Fester, K. Phytochemical Characterization and In Vitro Anti-Inflammatory, Antioxidant and Antimicrobial Activity of Combretum Collinum Fresen Leaves Extracts from Benin. Molecules 2020, 25, 288.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop