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Open AccessArticle

Phytochemical Characterization and In Vitro Anti-Inflammatory, Antioxidant and Antimicrobial Activity of Combretum Collinum Fresen Leaves Extracts from Benin

Institute of Pharmacy, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany
Institute for Medical Physics and Biophysics, Leipzig University, Härtelstr 16-18, 04107 Leipzig, Germany
Antimicrobial Agents Unit, Fraunhofer Institute for Cell Therapy and Immunology, Perlickstraße 1, 04103 Leipzig, Germany
Institute of Analytical Chemistry, Leipzig University, Linnéstr 3, 04103 Leipzig, Germany
Inter-Regional University of Industrial Engineering Biotechnologies and Applied Sciences, IRGIB Africa University, Cotonou 07 BP 231, Benin
Faculty of Natural and Environmental Sciences, Zittau/Görlitz University of Applied Sciences, 02763 Zittau, Germany
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Molecules 2020, 25(2), 288;
Received: 4 December 2019 / Revised: 30 December 2019 / Accepted: 7 January 2020 / Published: 10 January 2020
(This article belongs to the Special Issue Biological Activities of Medicinal Plants)
Leaves from Combretum collinum Fresen (Combretaceae) are commonly used for the treatment of inflammatory conditions, wound healing and bacterial infections in traditional West African medicine. This research focuses on the characterization of the phenolic profile and lipophilic compounds of leaves extracts of C. collinum. Studies of the in vitro anti-inflammatory activity were performed in TNFα stimulated HaCaT cells and antibacterial activity was evaluated with agar well diffusion and microdilution assays. Antioxidant activity was determined by DPPH and ABTS assays and compared to standards. The phytochemical studies confirmed myricetin-3-O-rhamnoside and myricetin-3-O-glucoside as major components of the leaves extracts, each contributing significantly to the antioxidant activity of the hydrophilic extracts. GC-MS analysis identified 19 substances that were confirmed by comparison with spectral library data and authentic standards. Combretum collinum aqueous leaves extract decreased pro-inflammatory mediators in TNFα stimulated HaCaT cells. Further investigations showed that myricetin-3-O-rhamnoside has an anti-inflammatory effect on IL-8 secretion. In the antimicrobial screening, the largest inhibition zones were found against S. epidermidis, MRSA and S. aureus. MIC values resulted in 275.0 µg/mL for S. epidermidis and 385.5 µg/mL for MRSA. The in vitro anti-inflammatory, antibacterial and antioxidant activity supports topical use of C. collinum leaves extracts in traditional West African medicine. View Full-Text
Keywords: Combretum collinum; anti-inflammatory; antimicrobial; keratinocytes; HaCaT Combretum collinum; anti-inflammatory; antimicrobial; keratinocytes; HaCaT
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Marquardt, P.; Seide, R.; Vissiennon, C.; Schubert, A.; Birkemeyer, C.; Ahyi, V.; Fester, K. Phytochemical Characterization and In Vitro Anti-Inflammatory, Antioxidant and Antimicrobial Activity of Combretum Collinum Fresen Leaves Extracts from Benin. Molecules 2020, 25, 288.

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