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Open AccessArticle

Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils

Department of Pharmaceutical Biology, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
Department of Weed Science and Tillage Systems, Institute of Soil Science and Plant Cultivation State Research Institute, Orzechowa 61, 50-540 Wrocław, Poland
Department of Physical Chemistry, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland
Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland
Laboratory of Experimental Cultivation, Botanical Garden of Medicinal Plants, Wroclaw Medical University, Al. Jana Kochanowskiego 14, 50-556 Wroclaw, Poland
Author to whom correspondence should be addressed.
Academic Editors: Gianni Sacchetti and Gerold Jerz
Molecules 2020, 25(4), 842;
Received: 23 December 2019 / Revised: 26 January 2020 / Accepted: 13 February 2020 / Published: 14 February 2020
(This article belongs to the Special Issue Biological Activities of Medicinal Plants)
Due to certain differences in terms of molecular structure, isoquinoline alkaloids from Chelidonium majus engage in various biological activities. Apart from their well-documented antimicrobial potential, some phenanthridine and protoberberine derivatives as well as C. majus extract present with anti-inflammatory and cytotoxic effects. In this study, the LC–MS/MS method was used to determine alkaloids, phenolic acids, carboxylic acids, and hydroxybenzoic acids. We investigated five individually tested alkaloids (coptisine, berberine, chelidonine, chelerythrine, and sanguinarine) as well as C. majus root extract for their effect on the secretion of IL-1β, IL-8, and TNF-α in human polymorphonuclear leukocytes (neutrophils). Berberine, chelidonine, and chelerythrine significantly decreased the secretion of TNF-α in a concentration-dependent manner. Sanguinarine was found to be the most potent inhibitor of IL-1β secretion. However, the overproduction of IL-8 and TNF-α and a high cytotoxicity for these compounds were observed. Coptisine was highly cytotoxic and slightly decreased the secretion of the studied cytokines. The extract (1.25–12.5 μg/mL) increased cytokine secretion in a concentration-dependent manner, but an increase in cytotoxicity was also noted. The alkaloids were active at very low concentrations (0.625–2.5 μM), but their potential cytotoxic effects, except for chelidonine and chelerythrine, should not be ignored. View Full-Text
Keywords: isoquinoline alkaloids; cytokine secretion; cytotoxicity; greater celandine isoquinoline alkaloids; cytokine secretion; cytotoxicity; greater celandine
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Zielińska, S.; Czerwińska, M.E.; Dziągwa-Becker, M.; Dryś, A.; Kucharski, M.; Jezierska-Domaradzka, A.; Płachno, B.J.; Matkowski, A. Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils. Molecules 2020, 25, 842.

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