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Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides

Department of Health Science and Technology, Institute of Food, Nutrition and Health, ETH Zurich, 8092 Zurich, Switzerland
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Academic Editor: Gordon Morris
Molecules 2020, 25(13), 2982; https://doi.org/10.3390/molecules25132982
Received: 22 May 2020 / Revised: 21 June 2020 / Accepted: 24 June 2020 / Published: 29 June 2020
(This article belongs to the Special Issue Polysaccharides: Structure-Function Relationships)
The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated. Polysaccharides were treated at 50 °C with hydrochloric acid for 3–48 h. Portions of isopropanol (i-PrOH) were added sequentially to the hydrolyzates, resulting in fractions that were collected by centrifugation. As expected, a significant reduction of weight-average molecular weight (Mw) was observed with increasing hydrolysis time. Fractional precipitation was successfully applied to collect at least one polymer fraction with dispersity (Đ) close to one for each polysaccharide. The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected. Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides’ conformation. It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation. View Full-Text
Keywords: water-soluble polysaccharides; dietary fibers; partial acid hydrolysis; fractional precipitation with isopropanol; monosaccharide ratio; Mark-Houwink-Sakurada relationship water-soluble polysaccharides; dietary fibers; partial acid hydrolysis; fractional precipitation with isopropanol; monosaccharide ratio; Mark-Houwink-Sakurada relationship
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Lupo, C.; Boulos, S.; Nyström, L. Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides. Molecules 2020, 25, 2982.

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