Pretreatment of Anthocyanin from the Fruit of Vitis coignetiae Pulliat Acts as a Potent Inhibitor of TNF-α Effect by Inhibiting NF-κB-Regulated Genes in Human Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Anthocyanins Isolated from Meoru (AIM) Inhibited the Cell Proliferation, Tumor Necrosis Factor (TNF)-Augmented Cell Adhesion of MCF–7 Cells
2.2. Pre-Treatment of AIM Inhibits TNF-α Induced Metastasis Activity
2.3. TNF-α Induced Effect Was Reversed with the Treatment of AIM Prior by Suppression of NF-κB Regulated Proteins Involved in Proliferation, Invasion, and Angiogenesis
2.4. AIM Suppresses NF-κB Activity Partially Through Degradation of IκBα Phosphorylation
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Chemicals
4.2. AIM Preparation
4.3. Cell Proliferation Assays
4.4. Adhesion Assay
4.5. Cell Invasion Assay
4.6. Gelatin Zymography
4.7. Transfection
4.8. Luciferase Assay
4.9. Western Blotting
4.10. Statistics
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Paramanantham, A.; Kim, M.J.; Jung, E.J.; Nagappan, A.; Yun, J.W.; Kim, H.J.; Shin, S.C.; Kim, G.S.; Lee, W.S. Pretreatment of Anthocyanin from the Fruit of Vitis coignetiae Pulliat Acts as a Potent Inhibitor of TNF-α Effect by Inhibiting NF-κB-Regulated Genes in Human Breast Cancer Cells. Molecules 2020, 25, 2396. https://doi.org/10.3390/molecules25102396
Paramanantham A, Kim MJ, Jung EJ, Nagappan A, Yun JW, Kim HJ, Shin SC, Kim GS, Lee WS. Pretreatment of Anthocyanin from the Fruit of Vitis coignetiae Pulliat Acts as a Potent Inhibitor of TNF-α Effect by Inhibiting NF-κB-Regulated Genes in Human Breast Cancer Cells. Molecules. 2020; 25(10):2396. https://doi.org/10.3390/molecules25102396
Chicago/Turabian StyleParamanantham, Anjugam, Min Jeong Kim, Eun Joo Jung, Arulkumar Nagappan, Jeong Won Yun, Hye Jung Kim, Sung Chul Shin, Gon Sup Kim, and Won Sup Lee. 2020. "Pretreatment of Anthocyanin from the Fruit of Vitis coignetiae Pulliat Acts as a Potent Inhibitor of TNF-α Effect by Inhibiting NF-κB-Regulated Genes in Human Breast Cancer Cells" Molecules 25, no. 10: 2396. https://doi.org/10.3390/molecules25102396