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Open AccessArticle

Cornus macrophylla, the Antibacterial Activity of Organic Leaf Extracts and the Characterization of the More Lipophilic Components by GC/MS

Department of Botany, University of Gujrat, Gujrat 50700, Pakistan
Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, Khyber Pakhtunkhwa, Pakistan
Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus, Tobe Camp, Abbottabad 22060, Khyber Pakhtunkhwa, Pakistan
Author to whom correspondence should be addressed.
Academic Editors: Anna Andolfi, Maria Michela Salvatore and Francesca Mancianti
Molecules 2020, 25(10), 2395;
Received: 31 March 2020 / Revised: 27 April 2020 / Accepted: 3 May 2020 / Published: 21 May 2020
(This article belongs to the Special Issue Natural Secondary Metabolites)
In the present study, the antibacterial activity of Cornus macrophylla was examined. Organic solvent extracts of leaves were prepared using methanol, n-hexane, chloroform, and ethyl acetate. Antibacterial activity was examined by using a 100 mg/mL extract concentration. Penicillin was kept as a positive control while dimethyl sulfoxide was taken as a negative control. Methanolic extract exhibited a 21.5, 36.3, 25.3, and 23.7 mm inhibition zone diameter (IZD); n-hexane showed a 33, 40, 32.8, and 28.7 mm IZD; chloroform showed a 18.8, 29, 22.3, and 21.6 mm IZD; and ethyl acetate showed a 23.5, 30.2, 30, and 22.3 mm IZD against Erwinia carotovora, Pseudomonas syringae, Ralstonia solanacearum, and Xanthomonas axonopodis, respectively. The n-hexane extract revealed high antibacterial activity against all bacterial species as compared with methanolic, chloroform, and ethyl acetate extract. Gas Chromatography Mass Spectrometry (GC/MS) analysis of n-hexane extract depicted the presence of 55 compounds. Out of these compounds, one compound, identified as α-amyrin (Mol. wt = 426), exhibited the maximum peak area (32.64%), followed by A’-Neogammacer-22(29)-en-3-ol, acetate, (3.beta.,21.beta.)- (Mol. wt = 468) and β-amyrin (Mol. wt = 426) having peak areas of 25.97 and 6.77%, respectively. It was concluded that the antibacterial activity observed during the present investigation may be due to these compounds. View Full-Text
Keywords: α-amyrin; antibacterial activity; Cornus macrophylla; GC/MS α-amyrin; antibacterial activity; Cornus macrophylla; GC/MS
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Akbar, M.; Ali, U.; Khalil, T.; Iqbal, M.S.; Amin, A.; Naeem, R.; Nazir, A.; Waqas, H.M.; Aslam, Z.; Jafri, F.I.; Aslam, N.; Chohan, S.A. Cornus macrophylla, the Antibacterial Activity of Organic Leaf Extracts and the Characterization of the More Lipophilic Components by GC/MS. Molecules 2020, 25, 2395.

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