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Open AccessFeature PaperArticle

Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model

1
Department of Marine Life Science, Jeju National University, Jeju City 63243, Korea
2
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science and Technology, Jeju City 63349, Korea
3
Department of Seafood and Aquaculture Science, Gyeongsang National University, Tongyeong 53064, Korea
4
Marine Science Institute, Jeju National University, Jeju City 63333, Korea
5
Research Center for Industrial Development of Seafood, Gyeongsang National University, Tongyeong 53064, Korea
*
Authors to whom correspondence should be addressed.
Academic Editors: Yasuhito Shimada and Herman P. Spaink
Molecules 2020, 25(10), 2361; https://doi.org/10.3390/molecules25102361
Received: 17 April 2020 / Revised: 15 May 2020 / Accepted: 15 May 2020 / Published: 19 May 2020
(This article belongs to the Special Issue Zebrafish-Based Drug Screening)
Fucoidan is a fucose-enriched polysaccharide, obtained from brown algae, with demonstrated antioxidant properties. However, traditional extraction methods using water or chemical-based extraction methods have reduced yield and produced hazardous by-products. In this study, we isolated fucoidan at a high yield using enzyme-assisted extraction; the Celluclast enzyme assisted extract of Undaria pinnatifida sporophylls (FCUS). To examine the antioxidant properties of FCUS, oxidative stress was induced with 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH) in Vero cells and zebrafish model. FCUS was composed of 30.4% sulfate and 52.3% fucose. Pre-treatment of Vero cells with FCUS dose dependently inhibited AAPH-induced reactive oxygen species (ROS) production. Moreover, FCUS remarkably reduced cell death, ROS generation, and lipid peroxidation production in zebrafish larvae. Overall, these findings indicate that the sulfate-rich fucoidan of FCUS, obtained with an eco-friendly process, could be implemented as a beneficial antioxidant agent in the functional food industry. View Full-Text
Keywords: zebrafish; antioxidant; Undaria pinnatifida sporophylls; enzyme-assisted extraction; fucoidan zebrafish; antioxidant; Undaria pinnatifida sporophylls; enzyme-assisted extraction; fucoidan
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MDPI and ACS Style

Oh, J.-Y.; Kim, E.-A.; Kang, S.I.; Yang, H.-W.; Ryu, B.; Wang, L.; Lee, J.-S.; Jeon, Y.-J. Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model. Molecules 2020, 25, 2361.

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