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Open AccessArticle

Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology

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Chemistry Department, Universidad Nacional de Colombia, Bogotá, Carrera 45 No 26-85, Building 451, office 409, Bogotá 11321, Colombia
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Pharmacy Department, Universidad Nacional de Colombia, Bogotá Carrera 45 No 26-85, Building 450, Bogotá 11321, Colombia
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Author to whom correspondence should be addressed.
Academic Editor: Victoria Samanidou
Molecules 2019, 24(7), 1215; https://doi.org/10.3390/molecules24071215
Received: 28 February 2019 / Revised: 22 March 2019 / Accepted: 26 March 2019 / Published: 28 March 2019
(This article belongs to the Special Issue Solid Phase Extraction: State of the Art and Future Perspectives)
A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a new mathematical model based on its retention time was developed in order to predict the percentage of organic modifier in which the peptide will elute in RP-SPE. This information was used for designing the elution program of each molecule. It was possible to purify peptides with different physicochemical properties, showing that this method is versatile and requires low solvent consumption, making it the least polluting one. Reverse phase-SPE can easily be routinely implemented. It is an alternative to enrich and purified synthetic or natural molecules. View Full-Text
Keywords: peptide; solid phase extraction (SPE); preparative purification; gradient elution; solid phase peptide synthesis peptide; solid phase extraction (SPE); preparative purification; gradient elution; solid phase peptide synthesis
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MDPI and ACS Style

Insuasty Cepeda, D.S.; Pineda Castañeda, H.M.; Rodríguez Mayor, A.V.; García Castañeda, J.E.; Maldonado Villamil, M.; Fierro Medina, R.; Rivera Monroy, Z.J. Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology. Molecules 2019, 24, 1215.

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