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Article

Evaluation of the Anti-Trypanosomal Activity of Vietnamese Essential Oils, with Emphasis on Curcuma longa L. and Its Components

by
Thanh Binh Le
1,2,*,
Claire Beaufay
1,
Duc Trong Nghiem
3,
Tuan Anh Pham
2,
Marie-Paule Mingeot-Leclercq
4 and
Joëlle Quetin-Leclercq
1,*
1
GNOS Research Group, Louvain Drug Research Institute, Université catholique de Louvain, UCLouvain, 1200 Bruxelles, Belgium
2
Department of Pharmacognosy, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Hanoi 100000, Vietnam
3
Department of Botany, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Hanoi 100000, Vietnam
4
TFAR Research Group, Louvain Drug Research Institute, Université catholique de Louvain, UCLouvain, 1200 Bruxelles, Belgium
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(6), 1158; https://doi.org/10.3390/molecules24061158
Submission received: 12 February 2019 / Revised: 8 March 2019 / Accepted: 11 March 2019 / Published: 23 March 2019
(This article belongs to the Special Issue Biological Activities of Essential Oils)
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Abstract

Human African trypanosomiasis (HAT), known as sleeping sickness and caused by Trypanosoma brucei, is threatening low-income populations in sub-Saharan African countries with 61 million people at risk of infection. In order to discover new natural products against HAT, thirty-seven Vietnamese essential oils (EOs) were screened for their activity in vitro on Trypanosoma brucei brucei (Tbb) and cytotoxicity on mammalian cells (WI38, J774). Based on the selectivity indices (SIs), the more active and selective EOs were analyzed by gas chromatography. The anti-trypanosomal activity and cytotoxicity of some major compounds (isolated or commercial) were also determined. Our results showed for the first time the selective anti-trypanosomal effect of four EOs, extracted from three Zingiberaceae species (Curcuma longa, Curcuma zedoaria, and Zingiber officinale) and one Lauraceae species (Litsea cubeba) with IC50 values of 3.17 ± 0.72, 2.51 ± 1.08, 3.10 ± 0.08, and 2.67 ± 1.12 nL/mL respectively and SI > 10. Identified compounds accounted for more than 85% for each of them. Among the five major components of Curcuma longa EO, curlone is the most promising anti-trypanosomal candidate with an IC50 of 1.38 ± 0.45 µg/mL and SIs of 31.7 and 18.2 compared to WI38 and J774 respectively.

1. Introduction

Human African trypanosomiasis (HAT) or sleeping sickness is caused by two subspecies of the parasite Trypanosoma brucei, T. brucei gambiense and rhodesiense, while another subspecies, T. brucei brucei affects non-human vertebrates [1]. T. brucei gambiense causes the chronic form in West and Central Africa while T. brucei rhodesiense causes the acute form in Eastern and Southern Africa. Although many efforts were made this past decade to decrease HAT incidence, this fatal disease is still endemic in 36 African countries [2]. Remote rural areas are the most affected partly because of high poverty, higher risk of infection from the livestock reservoir (by the tsetse fly, responsible for parasite transmission, mainly living in rural areas), and lack of health care accessibility and infrastructures for current drugs administration [1]. Furthermore, it was reported recently that trypanosomes also have an extravascular localization [3,4] which makes it difficult to eliminate the disease. Moreover, available drugs for the treatment of HAT: pentamidine, suramine, melarsoprol, eflornithine, and nifurtimox, have shown not only a lot of serious side effects and limited efficacy but also the increase of drug resistance [5]. The newest product, fexinidazole, which was recommended by the European Medicines Agency in November 2018 as the first oral treatment for HAT, is however only active against infections caused by T. brucei gambiense [6]. So, research for alternative strategies is still needed.
Essential oils (EOs) along with other secondary metabolites extracted from plants have been used all over the world for various biological and pharmacological activities, such as antibacterial, anti-inflammatory, anti-fungal, anti-mutagenic, anti-cancer, and anti-oxidant [7]. Interestingly, EOs, due to their amphiphilic property and small molecular sizes, can cross the blood–brain barrier easily [8], which is essential to treat the neurological phase of the disease. This crossing of the blood–brain barrier constitutes a major limitation for pentamidine and suramin efficacy [9], but makes EOs become promising candidates in the development of new treatments. Indeed, a review from 2013 until April 2017 showed that 56 EOs were tested for anti-trypanosomal activity with 9 strongly and 20 moderately effective EOs [10].
However, EOs are very complex mixtures of different volatile compounds depending not only on the plant species, environmental conditions, and geographic variations, but also on other variables such as methods of harvesting, extraction, storage, and plant-related factors including parts of the plant and maturation of the plant [11]. A correct characterization of EO composition is therefore important for quality control but also for the study of the activity, toxicity, and mechanisms of action.
In the continuity of our anti-parasitic evaluation [12], thirty-seven EOs extracted from Vietnamese plants were investigated for their anti-trypanosomal activity against Trypanosoma brucei brucei (Tbb) bloodstream form. The more interesting samples were analyzed for their compositions and major components were then tested to identify active compounds.

2. Results

Based on previous criteria (IC50 < 2 µg/mL: strongly effective; IC50 between 2 and 20 µg/mL: moderately active) [13], the thirty-seven studied EOs were firstly screened at two concentrations of 50 and 25 nL/mL (1 nL is considered to be almost 1 µg depending on the density of the EO) to identify the most active samples. Four EOs, extracted from Cinnamomum cassia, Curcuma zedoaria, Dysphania ambrosioides, and Zingiber zerumbet, were however tested at lower concentrations, 10 and 5 nL/mL, because their very volatile constituents decreased the growth of control cells in the neighboring wells or plates in the oven at higher concentrations (data not shown). This “vapor effect” was already mentioned in the study of Behar et al. [14]. The percentages of viable parasites treated at 25 (or 10) nL/mL of EO are represented in Figure 1. Nineteen EOs were determined as promising candidates for further investigations, showing less than 3% of viable parasites at the lowest tested concentration.
These nineteen EOs were then analyzed for dose-response activity on Tbb bloodstream form and also on mammalian WI38 and J774 cells to calculate IC50 values and selectivity index (SI). Three samples extracted from three Zingiberaceae species, Curcuma longa, Curcuma zedoaria, and Zingiber officinale, and one sample extracted from a Lauraceae species, Litsea cubeba, showed the most active and selective effects with IC50 values of 3.17, 2.51, 3.10, and 2.67 nL/mL respectively and SI > 10 compared to cytotoxicity (Table 1 in bold).
The chemical composition of these four interesting EOs was analyzed using gas chromatography (GC) with mass spectrometry (MS) and flame ionization detector (FID) in order to control their quality but also to identify some active compounds. As shown in Table 2, more than 85% of each EO composition was characterized. Monoterpenes such as citronellal (43.10%), isopulegol (11.10%), limonene (8.72%), pulegol (6.52%), linalool (5.60%), and citronellol (5.17%) were major components of L. cubeba EO while EOs of the three Zingiberaceae species contained mainly sesquiterpenes. Interestingly, α-zingiberene, β-bisabolene, β-sesquiphellandrene, and ar-curcumene were identified in both EOs extracted from C. longa and Z. officinale with relative percentages respectively of 25.38, 3.38, 18.27, and 5.22% (C. longa) and 27.71, 7.27, 8.08, and 2.71% (Z. officinale). The difference in the composition of these two EOs is that oxygenated sesquiterpenes (i.e., α-turmerone (10.28%), germacrone (3.34%), curlone (5.15%), and ar-turmerone (9.93%)) were found in C. longa EO while monoterpenes including β-phellandrene (14.78%) and camphene (6.94%) were found in Z. officinale EO. 8,9-Dehydro-9-formyl cycloisolongifolene (29.31%), curdione (13.52%), and germacrone (8.95%) were shown as major components of the EO extracted from another curcuma species, C. zedoaria.
The C. longa EO was chosen for further investigations because it was easy to obtain in a high amount as being present in the marc after turmeric starch extraction. The first fractionation using column chromatography with silica gel and gradients of eluents (n-hexane-ethyl acetate) allowed to obtain two important groups, CF1 with sesquiterpenes and CF5 with oxygenated sesquiterpenes. After the second column chromatography using silver nitrate impregnated silica gel of both fractions, three compounds, β-sesquiphellandrene, ar-curcumene, and curlone with a purity respectively of 96.9, 97.4, and 91.7%, were purified.
These isolated compounds along with two commercially available ones, α-zingiberene and ar-turmerone (chemical structures in Figure 2), were analyzed for anti-trypanosomal activity and cytotoxicity. The results are summarized in Table 3. Curlone with an IC50 of 1.38 µg/mL (6.32 µM) against Tbb bloodstream form and SI > 10 compared to cytotoxicity on mammalian cells could explain a part of the observed activity of the EO (IC50 = 3.17 nL/mL). This compound may be considered as a promising model for the development of a new treatment of HAT.

3. Discussion

This is the first time that these thirty-seven EOs extracted from Vietnamese plants were described for anti-trypanosomal activity in vitro. Some of these EOs were already reported to be tested on the same model, such as D. ambrosioides, M. alternifolia, and O. gratissimum [17,18,19], but they were extracted from plants collected in other countries with possibly different compositions. Based on a preliminary screening, half of them showed a potential activity on Tbb with less than 3% of viable parasites at 25 nL/mL (or 10 nL/mL for four of them). Within these nineteen EOs, the one extracted from C. cassia revealed the strongest effect (IC50 = 1.77 ± 0.15 nL/mL), 17 EOs showed moderate activity with IC50 values between 2–20 nL/mL, and one EO extracted from P. indica showed less interesting activity with an IC50 value of 21.29 ± 1.38 nL/mL. In order to identify the most selective EOs, the cytotoxicity on two different mammalian cell lines, WI38 and J774, was evaluated in parallel. Four samples extracted from C. longa, C. zedoaria, L. cubeba, and Z. officinale displayed SI from 14 to > 19 (WI38) and 11 to > 19 (J774). GC analyses led to the identification of more than 85% of their components. We observed the predominance of sesquiterpenes in EOs extracted from rhizomes of the three Zingiberaceae species (C. longa, C. zedoaria, and Z. officinale) while monoterpenes were the major compounds in the EO extracted from fruits of a Lauraceae species (L. cubeba).
In the literature, monoterpenes were already shown to be the major compounds of EOs extracted from L. cubeba, although the identified components were different. Indeed, EOs extracted from fruits of L. cubeba collected in China and Taiwan contained mostly citral (neral and geranial) from 57% to 81% [20,21,22,23,24,25]. The EO extracted from another sample collected in China contained limonene oxide (60%) and limonene (12%) [26]. In our study, the EO extracted from fruits of a Vietnamese sample of L. cubeba was dominated by six major compounds, citronellal (43.10%), isopulegol (11.10%), limonene (8.72%), pulegol (6.52%), linalool (5.60%), and citronellol (5.17%). This profile is in agreement with the one extracted from L. cubeba collected in India [27]. Four of these six compounds, citronellal, limonene, linalool, and citronellol, are already known for their anti-trypanosomal activity (IC50 = 2.76 ± 1.55 [28], 4.24 ± 1.27 [28] or 5.6 ± 1.6 [29], 2.5 [30] and 6.45 ± 4.86 µg/mL [28] respectively on Tbb bloodstream form). So, they can explain a part of the observed activity of L. cubeba EO (IC50 = 2.67 ± 1.12 nL/mL). Nevertheless, the activity of this EO may also partially be due to other compounds or a synergy between these components. It is worth noting that these compounds did not show any cytotoxicity at the highest tested concentration (50 µg/mL for citronellal and citronellol and 100 µg/mL for limonene and linalool) against different mammalian cell lines: CHO, WI38, Balb/3T3 fibroblast, and J774 in those studies [28,29,30].
The C. zedoaria EO composition including 8,9-dehydro-9-formyl cycloisolongifolene (29.31%), curdione (13.52%), and germacrone (8.95%) differed from both other EOs but also from previous articles. These publications reported the presence in higher amounts of either monoterpenes such as eucalyptol, p-cymene, α-phellandrene, and camphor or other sesquiterpenes such as curzerene, epicurzerene, curdione, curzerenone, and germacrone, depending on the analyzed samples [31]. This variability can be related to the different geographic origins but also to different chemotypes. Germacrone, one of the major compounds identified in our EO, did not show any effect on Tbb bloodstream form in the study of Petrelli et al. (IC50 > 100 µg/mL) [32], meaning that other components should be responsible for the observed activity of the Vietnamese C. zedoaria EO (IC50 = 2.51 ± 1.08 nL/mL). Knowing that this EO activity is moderately selective compared to the cytotoxicity on two mammalian cell lines, and that the plant is not difficult to cultivate, C. zedoaria EO and its components should be further studied.
The sesquiterpenes identified in the EO extracted from Z. officinale rhizomes were α-zingiberene (27.71%), β-sesquiphellandrene (8.08%), β-bisabolene (7.27%), α-farnesene (3.71%), and ar-curcumene (2.71%). Some monoterpenes were also found, such as β-phellandrene (14.78%), camphene (6.94%), and neral (3.16%). This composition is similar to most reports on Z. officinale EOs, including samples collected in Brazil, Burkina Faso, Iran, Pakistan, or São Tomé and Príncipe [33,34,35,36,37]. However, rhizomes of samples collected in Australia, India, or Thailand showed a higher quantity of two monoterpenes, citral (neral and geranial) and camphene [38,39,40]. Concerning the anti-trypanosomal activity of these major components, only camphene was tested in the study of Mulyaningsih et al. However its activity was not significant against Tbb bloodstream form (IC50 value of 80.66 ± 0.87 μg/mL) [41].
Identified compounds of Vietnamese C. longa EO were in agreement with the reported chemical profile of samples collected from other countries (e.g. Malaysia, China, India, Pakistan, Bhutan, Brazil, Nigeria, Cameroon, and France) [31]. It contains mainly sesquiterpenes: α-zingiberene—25.38%, β-sesquiphellandrene—18.27%, α-turmerone—10.28%, ar-turmerone—9.93%, ar-curcumene—5.22%, curlone—5.15%, β-bisabolene—3.38%, and germacrone—3.34%. Regarding the anti-trypanosomal activity of C. longa, previous reports only focused on curcumin and other curcuminoids with an IC50 around 5 μM for curcumin [42], 9.84 ± 0.84 μM for bisdemethoxycurcumin, and 7.19 ± 1.02 μM for demethoxycurcumin [43]. We report here for the first time the interesting effect of the C. longa EO against Tbb bloodstream form (IC50 = 3.17 ± 0.72 nL/mL) with a good selectivity (SI > 10). Thanks to the purification process, β-sesquiphellandrene, ar-curcumene, and curlone could be isolated and tested for anti-trypanosomal activity together with two commercially available compounds, α-zingiberene and ar-turmerone. Among them, curlone revealed the most interesting effect against Tbb bloodstream form with an IC50 value of 1.38 ± 0.45 µg/mL (6.32 ± 2.38 µM) and SI of 31.7 and 18.2 compared to cytotoxicity on two mammalian cell lines, WI38 and J774, respectively. This result showed the potential of curlone in the research for new anti-trypanosomal molecules. α-Zingiberene, β-sesquiphellandrene, and ar-curcumene showed a moderate effect with IC50 values of 6.91 ± 2.60, 9.89 ± 1.18, and 13.38 ± 2.46 µg/mL respectively. However their activity along with curlone can explain a part of the observed activity for the C. longa EO.
Concerning the mechanism of action of curlone, which is not commercially available, there are no data in the literature. Three other related sesquiterpenoids, α-zingiberene, β-sesquiphellandrene, and ar-turmerone, showed apoptotic effects on different human cancer cells which was associated with the release of mitochondrial cytochrome c and the activation of capase-3 at concentrations of 120 and 160 µg/mL (α-zingiberene), 5.10 µg/mL (β-sesquiphellandrene), and 40, 80, and 120 µg/mL (ar-turmerone) [44,45,46]. The structure of curlone shows the presence of both an exocyclic ethylene group as in β-sesquiphellandrene and a ketone group as in ar-turmerone. This suggests that these two groups may play an important role in anti-trypanosomal activity of the compound.
As mentioned before, it is very important to emphasize the composition complexity of EOs, making it difficult to identify active compounds [11]. Indeed, in these mixtures, most components were found in low percentages, while two or three accounted for 20%–70% of the whole oil [47]. Major compounds can be studied easier and are often considered as responsible for EO biological activities. However other constituents could of course contribute to the activity through a higher efficacy, additional and/or synergistic activity [48]. For example, eucalyptol, which was characterized as the major component (accounts from 27% to 55%) in four of our tested EOs: A. aromaticum, E. blanda, H. coronarium, and L. cubeba (leaves) (data not shown), was not effective on Tbb as shown by its high IC50 value (83.15 µg/mL) [49]. However these EOs showed a moderate effect in this study with IC50 values in the range of 8–16 nL/mL. On the contrary, antagonistic effects may also occur between constituents of these mixtures [48]. An interesting example is terpinolene, which accounts for 55% of our C. indica EO (data not shown). This compound was shown to have a very strong activity against Tbb bloodstream form (IC50 = 0.035 ± 0.05 μg/mL or 0.041 nL/mL) [50], however the IC50 value of our C. indica EO was 330 times higher (13.22 ± 4.54 nL/mL). The difference in the experiment design could also be another explanation.

4. Materials and Methods

4.1. Chemicals and Materials

ar-Curcumene, curlone, and β-sesquiphellandrene were isolated from C. longa EO; ar-turmerone (purity of 97.9 %-GC) was purchased from Sigma-Aldrich (Bornem, Belgium); α-zingiberene (purity of 95 %-TLC) was acquired from Santa Cruz Biotechnology (Heidelberg, Germany).
Column chromatography (CC) was performed using silica gel 60 (70–230 mesh) (Merck KGaA, Darmstadt, Germany). Thin-layer chromatography (TLC) analysis was done on a sheet pre-coated with silica gel (Merck KGaA, Darmstadt, Germany) and impregnated with AgNO3 as described by Sliwowski [51]. The GC-MS analyses were carried out on a TRACE GC 2000 series (Thermo-Quest, Rodano, Italy) and the GC-FID analyses were done on a FOCUS GC (Thermo Finnigan, Milan, Italy).
All used organic solvents were HPLC grade (VWR, Leuven, Belgium).
1H-NMR and 13C-NMR spectra were recorded in CDCl3 on a Bruker Avance spectrometer (Wissembourg, France) at 400 and 100 MHz respectively.
In the anti-trypanosomal and cytotoxicity assays, alamar blue was obtained from Thermo Fisher Scientific (Merelbeke, Belgium); tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), suramin, and camptothecin were obtained from Sigma-Aldrich (Bornem, Belgium). The fluorescence or absorbance was measured on a spectrophotometer (SpectraMax-Molecular Devices, Berkshire, UK).

4.2. PLANTS Collection and Essential Oils Extraction

The thirty-seven EOs used in this study were extracted from Vietnamese plants as described previously [12]. All EOs were dissolved in dimethyl sulfoxide (DMSO) to obtain stock solutions at 20 µL/mL and then further diluted in fresh medium for anti-trypanosomal and cytotoxicity assays.

4.3. Parasites, Cells, and Media

Trypanosoma brucei brucei, although not infecting humans because of its susceptibility to the innate immune system, has been used as a good predictive model in a first screening for the identification of anti-trypanosomal compounds [52,53]. Bloodstream forms of this parasite (Tbb, strain 427) were grown in HMI-9 medium (IMDM-Gibco-Thermo Fisher Scientific, Merelbeke, Belgium-supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich, Bornem, Belgium) and bloodstream form supporting factors) [54].
The human non-cancer fibroblast cell line WI38 (ATCC Number CCL-75 – Standards, UK) and macrophage-like murine cell line J774 (ECACC Number 91051511 – Public Health England, UK) were grown in DMEM and RPMI medium (Gibco-Thermo Fisher Scientific, Merelbeke, Belgium or Sigma-Aldrich, Bornem, Belgium), respectively, supplemented with 10% fetal bovine serum and penicillin-streptomycin (100 UI/mL) (Sigma-Aldrich, Bornem, Belgium).
Parasites and cells cultures were maintained at 37 °C in 5% CO2 incubator.

4.4. Anti-Trypanosomal Assay

The assay was performed in 96-well plates as previously described [28]. The primary screening was repeated two times in triplicate at the concentrations of 50 nL/mL and 25 nL/mL (for 33 EOs) or 10 nL/mL and 5 nL/mL (for 4 EOs). Fifty µL of parasite culture (5 x 104 parasites/mL) was added with 50 µL of diluted EOs in each well. Ten µL of alamar blue (diluted with PBS at the ratio 1:1) was added to each well after 72 h of incubation and the plates were further incubated for 4 h. The fluorescence of the reduced reagent was measured on a spectrophotometer at 530 nm excitation and 590 nm emission wavelengths. Suramin was used as positive control. The EOs that inhibited more than 50% of the parasite growth at 25 or 10 nL/mL were analyzed for IC50 determination. Samples were tested in eight serial three-fold dilutions ranging from 50–0.02 nL/mL, except Cinnamomum cassia EO, Curcuma zedoaria EO, and Zingiber zerumbet EO (ranging from 10–0.005 nL/mL) and Dysphania ambrosioides EO (ranging from 5–0.002 nL/mL) in duplicate. IC50 values were calculated from dose response growth inhibition curves using Microsoft Excel files and mean IC50 values were obtained from at least three repetitions.

4.5. Cytotoxicity Assay

The cytotoxicity assays were performed as described previously [12] with concentrations ranging from 50 to 1.40 nL/mL (dilution of 1.67). The selectivity index (SI) values were calculated using the formula:
SI = IC50 on mammalian cells/IC50 on protozoan parasites

4.6. Essential Oils Analysis

Four EOs were analyzed as explained in our previous publication [12].

4.7. Components Isolation

C. longa EO obtained by hydro-distillation was subjected to column chromatography on silica gel 60 (70–230 mesh) using n-hexane/ethyl acetate (EtOAc) gradients as the eluent to yield six fractions (CF1-CF6). CF1 and CF5 were further separated to obtain three compounds by column chromatography using AgNO3-impregnated silica gel as stationary phase because argentation chromatography is known for the purification of cis-trans-isomers or positional isomers mixtures [55,56,57,58]. This separation relies on the weak interactions between silver ions and the π-orbital of olefins in which cis-olefinic structures complex more tightly with silver ions than the trans-isomers [55]. We modified the procedure of Denyer et al. [58] with 10% (w/w) of silver nitrate instead of 25%, and a gradient of n-hexane and toluene was preferred to hexane and benzene (Figure 3). These isolated compounds were confirmed as β-sesquiphellandrene, ar-curcumene, and curlone using NMR in comparison with data from previous reports [59,60,61] and their purity was checked by GC-FID.

5. Conclusions

Our results highlighted for the first time the interesting anti-trypanosomal activity of four EOs extracted from Vietnamese plants, C. longa, C. zedoaria, L. cubeba, and Z. officinale. Monoterpenes were major components of L. cubeba EOs, while the three other EOs contained mostly sesquiterpenes. Among the five major compounds of the C. longa EO, curlone was the most active and selective compound. This compound can explain a part of the observed activity of the EO. Its activity should be further investigated in the research for new anti-trypanosomal compounds.

Author Contributions

Conceptualization, M.-P.M.-L. and J.Q.-L.; formal analysis, T.B.L. and C.B.; investigation, T.B.L., C.B., and T.A.P.; resources, D.T.N.; supervision, M.-P.M.-L. and J.Q.-L.; writing-original draft, T.B.L.; writing-review and editing, C.B., D.T.N., T.A.P., M.-P.M.-L., and J.Q.-L.

Funding

This research was funded by VIETNAMESE MINISTRY OF EDUCATION AND TRAINING, grant number 100/QĐ-BGDĐT, and BELGIAN NATIONAL FUND FOR SCIENTIFIC RESEARCH (FNRS), grant number PDR T.0190.13.

Acknowledgments

We wish to thank Marie-Christine Fayt and Marie-France Hérent for skillful technical assistance in GC, Maude Bourlet for helping on cells and parasites cultivation, and all the members of the Pharmacognosy group from LDRI, UCLouvain, Belgium, for helpful scientific advice and support.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Centers for Disease Control and Prevention (CDC). Parasites—Sleeping Sickness—Epidemiology & Risk Factors; CDC: Atlanta, GA, USA. Available online: https://www.cdc.gov/parasites/sleepingsickness/epi.html (accessed on 23 January 2019).
  2. Drugs for Neglected Diseases initiative (DNDi). Diseases & Projects—Sleeping Sickness—Fact Sheet. DNDi: Geneva, Switzerland. Available online: https://www.dndi.org/wp-content/uploads/2018/12/Factsheet2018_HAT.pdf (accessed on 23 January 2019).
  3. Capewell, P.; Cren-Travaillé, C.; Marchesi, F.; Johnston, P.; Clucas, C.; Benson, R.A.; Gorman, T.A.; Calvo-Alvarez, E.; Crouzols, A.; Jouvion, G.; et al. The skin is a significant but overlooked anatomical reservoir for vector-borne African trypanosomes. Elife 2016, 5, e17716. [Google Scholar] [CrossRef]
  4. Trindade, S.; Rijo-Ferreira, F.; Carvalho, T.; Pinto-Neves, D.; Guegan, F.; Aresta-Branco, F.; Bento, F.; Young, S.A.; Pinto, A.; Van Den Abbeele, J.; et al. Trypanosoma brucei Parasites Occupy and Functionally Adapt to the Adipose Tissue in Mice. Cell Host Microbe 2016, 19, 837–848. [Google Scholar] [CrossRef] [PubMed]
  5. World Health Organization (WHO). Human African Trypanosomiasis—The Disease—Symptoms, Diagnosis and Treatment; WHO: Geneva, Switzerland; Available online: http://www.who.int/trypanosomiasis_african/disease/diagnosis/en/ (accessed on 23 January 2019).
  6. Drugs for Neglected Diseases initiative (DNDi). Diseases & Projects—Portfolio—Fexinidazole (HAT). DNDi: Geneva, Switzerland. Available online: https://www.dndi.org/diseases-projects/portfolio/fexinidazole/ (accessed on 23 January 2019).
  7. Chiara Cristiano, M.; Cosco, D.; Paolino, D. Technological Aspects of Essential Oils. In Aromatherapy: Basic Mechanisms and Evidence-Based Clinical Use; Bagetta, G., Cosentino, M., Sakurada, T., Eds.; CRC Press: Boca Raton, FL, USA, 2016; pp. 152–153. ISBN 978-1-4822-4663-6. [Google Scholar]
  8. Heuberger, E. Effects of Essential Oils in the Central Nervous System. In Handbook of Essential Oils: Science, Technology and Applications; Can Baser, K.H., Buchbauer, G., Eds.; CRC Press: Boca Raton, FL, USA, 2010; p. 283. ISBN 978-1-4200-6315-8. [Google Scholar]
  9. World Health Organization (WHO). Human African Trypanosomiasis—African Trypanosomiasis—Drugs; WHO: Geneva, Switzerland; Available online: http://www.who.int/trypanosomiasis_african/drugs/en/ (accessed on 23 January 2019).
  10. Le, T.B.; Beaufay, C.; Bonneau, N.; Mingeot-Leclercq, M.-P.; Quetin-Leclercq, J. Anti-protozoal activity of essential oils and their constituents against Leishmania, Plasmodium and Trypanosoma. Phytochimie 2018, 1, 1–33. [Google Scholar]
  11. Montoro, P.; Masullo, M.; Piacente, S.; Pizza, C. Extraction, Sample Preparation, and Analytical Methods for Quality Issues of Essential Oils. In Aromatherapy: Basic Mechanisms and Evidence-Based Clinical Use; Bagetta, G., Cosentino, M., Sakurada, T., Eds.; CRC Press: Boca Raton, FL, USA, 2016; p. 153. ISBN 978-1-4822-4663-6. [Google Scholar]
  12. Le, T.B.; Beaufay, C.; Nghiem, D.T.; Mingeot-Leclercq, M.-P.; Quetin-Leclercq, J. In vitro anti-leishmanial activity of essential oils extracted from Vietnamese plants. Molecules 2017, 22, 1071. [Google Scholar] [CrossRef] [PubMed]
  13. Bero, J.; Kpoviessi, S.; Quetin-Leclercq, J. Anti-Parasitic Activity of Essential Oils and their Constituents against Plasmodium, Trypanosoma and Leishmania. In Novel Plant Bioresource: Applications in Food, Medicine and Cosmetic; Gurib-Fakim, A., Ed.; John Wiley & Sons: Oxford, UK, 2014; pp. 455–469. ISBN 978-1-118-46061-0. [Google Scholar]
  14. Behar, R.Z.; Davis, B.; Wang, Y.; Bahl, V.; Lin, S.; Talbot, P. Identification of toxicants in cinnamon-flavored electronic cigarette refill fluids. Toxicol. In Vitro 2014, 28, 198–208. [Google Scholar] [CrossRef]
  15. Hérent, M.F.; De Bie, V.; Tilquin, B. Determination of new retention indices for quick identification of essential oils compounds. J. Pharm. Biomed. Anal. 2007, 43, 886–892. [Google Scholar] [CrossRef] [PubMed]
  16. Babushok, V.I.; Linstrom, P.J.; Zenkevich, I.G. Retention Indices for Frequently Reported Compounds of Plant Essential Oils. J. Phys. Chem. Ref. Data 2011, 40, 043101. [Google Scholar] [CrossRef]
  17. Monzote, L.; García, M.; Pastor, J.; Gil, L.; Scull, R.; Maes, L.; Cos, P.; Gille, L. Essential oil from Chenopodium ambrosioides and main components: Activity against Leishmania, their mitochondria and other microorganisms. Exp. Parasitol. 2014, 136, 20–26. [Google Scholar] [CrossRef] [PubMed]
  18. Cheikh-Ali, Z.; Adiko, M.; Bouttier, S.; Bories, C.; Okpekon, T.; Poupon, E.; Champy, P. Composition, and antimicrobial and remarkable antiprotozoal activities of the essential oil of rhizomes of Aframomum sceptrum K. Schum. (Zingiberaceae). Chem. Biodivers. 2011, 8, 658–667. [Google Scholar] [CrossRef] [PubMed]
  19. Kpadonou Kpoviessi, B.G.H.; Kpoviessi, S.D.S.; Yayi Ladekan, E.; Gbaguidi, F.; Frédérich, M.; Moudachirou, M.; Quetin-Leclercq, J.; Accrombessi, G.C.; Bero, J. In vitro antitrypanosomal and antiplasmodial activities of crude extracts and essential oils of Ocimum gratissimum Linn from Benin and influence of vegetative stage. J. Ethnopharmacol. 2014, 155, 1417–1423. [Google Scholar] [CrossRef] [PubMed]
  20. Si, L.; Chen, Y.; Han, X.; Zhan, Z.; Tian, S.; Cui, Q.; Wang, Y. Chemical composition of essential oils of Litsea cubeba harvested from its distribution areas in China. Molecules 2012, 17, 7057–7066. [Google Scholar] [CrossRef] [PubMed]
  21. Wang, H.; Liu, Y. Chemical composition and antibacterial activity of essential oils from different parts of Litsea cubeba. Chem. Biodivers. 2010, 7, 229–235. [Google Scholar] [CrossRef] [PubMed]
  22. Huang, X.W.; Feng, Y.C.; Huang, Y.; Li, H.L. Potential cosmetic application of essential oil extracted from Litsea cubeba fruits from China. J. Essent. Oil Res. 2013, 25, 112–119. [Google Scholar] [CrossRef]
  23. Liu, T.T.; Yang, T.S. Antimicrobial impact of the components of essential oil of Litsea cubeba from Taiwan and antimicrobial activity of the oil in food systems. Int. J. Food Microbiol. 2012, 156, 68–75. [Google Scholar] [CrossRef] [PubMed]
  24. Chen, H.C.; Chang, W.T.; Hseu, Y.C.; Chen, H.Y.; Chuang, C.H.; Lin, C.C.; Lee, M.S.; Lin, M.K. Immunosuppressive effect of Litsea cubeba L. essential oil on dendritic cell and contact hypersensitivity responses. Int. J. Mol. Sci. 2016, 17, 1319. [Google Scholar] [CrossRef] [PubMed]
  25. Yang, T.S.; Liou, M.L.; Hu, T.F.; Peng, C.W.; Liu, T.T. Antimicrobial activity of the essential oil of Litsea cubeba on cariogenic bacteria. J. Essent. Oil Res. 2013, 25, 120–128. [Google Scholar] [CrossRef]
  26. Li, Y.; Kong, W.; Li, M.; Liu, H.; Zhao, X.; Yang, S.; Yang, M. Litsea cubeba essential oil as the potential natural fumigant: Inhibition of Aspergillus flavus and AFB1 production in licorice. Ind. Crops Prod. 2016, 80, 186–193. [Google Scholar] [CrossRef]
  27. Saikia, A.K.; Chetia, D.; Darrigo, M.; Smeriglio, A.; Strano, T.; Ruberto, G. Screening of fruit and leaf essential oils of Litsea cubeba Pers. from north-east India—Chemical composition and antimicrobial activity. J. Essent. Oil Res. 2013, 25, 330–338. [Google Scholar] [CrossRef]
  28. Kpoviessi, S.; Bero, J.; Agbani, P.; Gbaguidi, F.; Kpadonou-Kpoviessi, B.; Sinsin, B.; Accrombessi, G.; Frédérich, M.; Moudachirou, M.; Quetin-Leclercq, J. Chemical composition, cytotoxicity and in vitro antitrypanosomal and antiplasmodial activity of the essential oils of four Cymbopogon species from Benin. J. Ethnopharmacol. 2014, 151, 652–659. [Google Scholar] [CrossRef]
  29. Petrelli, R.; Orsomando, G.; Sorci, L.; Maggi, F.; Ranjbarian, F.; Biapa Nya, P.C.; Petrelli, D.; Vitali, L.A.; Lupidi, G.; Quassinti, L.; et al. Biological activities of the essential oil from Erigeron floribundus. Molecules 2016, 21, 1065. [Google Scholar] [CrossRef] [PubMed]
  30. Hoet, S.; Stévigny, C.; Hérent, M.-F.; Quetin-Leclercq, J. Antitrypanosomal Compounds from the Leaf Essential Oil of Strychnos spinosa. Planta Med. 2006, 72, 480–482. [Google Scholar] [CrossRef] [PubMed]
  31. Dosoky, N.S.; Setzer, W.N. Chemical composition and biological activities of Essential Oils of Curcuma Species. Nutrients 2018, 10, 1196. [Google Scholar] [CrossRef] [PubMed]
  32. Petrelli, R.; Ranjbarian, F.; Dall’Acqua, S.; Papa, F.; Iannarelli, R.; Ngahang Kamte, S.L.; Vittori, S.; Benelli, G.; Maggi, F.; Hofer, A.; et al. An overlooked horticultural crop, Smyrnium olusatrum, as a potential source of compounds effective against African trypanosomiasis. Parasitol. Int. 2017, 66, 146–151. [Google Scholar] [CrossRef] [PubMed]
  33. Yamamoto-Ribeiro, M.M.G.; Grespan, R.; Kohiyama, C.Y.; Ferreira, F.D.; Mossini, S.A.G.; Silva, E.L.; De Abreu Filho, B.A.; Mikcha, J.M.G.; Machinski Junior, M. Effect of Zingiber officinale essential oil on Fusarium verticillioides and fumonisin production. Food Chem. 2013, 141, 3147–3152. [Google Scholar] [CrossRef] [PubMed]
  34. Bayala, B.; Bassole, I.H.N.; Gnoula, C.; Nebie, R.; Yonli, A.; Morel, L.; Figueredo, G.; Nikiema, J.B.; Lobaccaro, J.M.A.; Simpore, J. Chemical composition, antioxidant, anti-inflammatory and anti-proliferative activities of essential oils of plants from Burkina Faso. PLoS ONE 2014, 9, e92122. [Google Scholar] [CrossRef]
  35. Noori, S.; Zeynali, F.; Almasi, H. Antimicrobial and antioxidant efficiency of nanoemulsion-based edible coating containing ginger (Zingiber officinale) essential oil and its effect on safety and quality attributes of chicken breast fillets. Food Control 2018, 84, 312–320. [Google Scholar] [CrossRef]
  36. El-Ghorab, A.H.; Nauman, M.; Anjum, F.M.; Hussain, S.; Nadeem, M. A comparative study on chemical composition and antioxidant activity of ginger (Zingiber officinale) and cumin (Cuminum cyminum). J. Agric. Food Chem. 2010, 58, 8231–8237. [Google Scholar] [CrossRef] [PubMed]
  37. Martins, A.P.; Salgueiro, L.; Gonçalves, M.J.; da Cunha, A.P.; Vila, R.; Cañigueral, S.; Mazzoni, V.; Tomi, F.; Casanova, J. Essential oil composition and antimicrobial activity of three Zingiberaceae from S Tomé e Príncipe. Planta Med. 2001, 67, 580–584. [Google Scholar] [CrossRef] [PubMed]
  38. Wohlmuth, H.; Smith, M.K.; Brooks, L.O.; Myers, S.P.; Leach, D.N. Essential oil composition of diploid and tetraploid clones of ginger (Zingiber officinale Roscoe) grown in Australia. J. Agric. Food Chem. 2006, 54, 1414–1419. [Google Scholar] [CrossRef]
  39. Singh, G.; Kapoor, I.P.S.; Singh, P.; de Heluani, C.S.; de Lampasona, M.P.; Catalan, C.A.N. Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food Chem. Toxicol. 2008, 46, 3295–3302. [Google Scholar] [CrossRef] [PubMed]
  40. Buddhakala, N.; Talubmook, C.; Sriyotha, P.; Wray, S.; Kupittayanant, S. Inhibitory effects of ginger oil on spontaneous and PGF2α- induced contraction of rat myometrium. Planta Med. 2008, 74, 385–391. [Google Scholar] [CrossRef]
  41. Mulyaningsih, S.; Youns, M.; El-Readi, M.Z.; Ashour, M.L.; Nibret, E.; Sporer, F.; Herrmann, F.; Reichling, J.; Wink, M. Biological activity of the essential oil of Kadsura longipedunculata (Schisandraceae) and its major components. J. Pharm. Pharmacol. 2010, 62, 1037–1044. [Google Scholar] [CrossRef] [PubMed]
  42. Haddad, M.; Sauvain, M.; Deharo, E. Curcuma as a parasiticidal agent: A review. Planta Med. 2011, 77, 672–678. [Google Scholar] [CrossRef]
  43. Sun, Y.N.; No, J.H.; Lee, G.Y.; Li, W.; Yang, S.Y.; Yang, G.; Schmidt, T.J.; Kang, J.S.; Kim, Y.H. Phenolic constituents of medicinal plants with activity against Trypanosoma brucei. Molecules 2016, 21, 480. [Google Scholar] [CrossRef]
  44. Lee, Y. Cytotoxicity Evaluation of Essential Oil and its Component from Zingiber officinale Roscoe. Toxicol. Res. 2016, 32, 225–230. [Google Scholar] [CrossRef] [PubMed]
  45. Tyagi, A.K.; Prasad, S.; Yuan, W.; Li, S.; Aggarwal, B.B. Identification of a novel compound (β-sesquiphellandrene) from turmeric (Curcuma longa) with anticancer potential: Comparison with curcumin. Investig. New Drugs 2015, 33, 1175–1186. [Google Scholar] [CrossRef] [PubMed]
  46. Lee, Y. Activation of apoptotic protein in U937 cells by a component of turmeric oil. BMB Rep. 2009, 42, 96–100. [Google Scholar] [CrossRef]
  47. Russo, R.; Corasaniti, M.T.; Bagetta, G.; Morrone, L.A. Essential Oils Exploited in Cytotoxicity Studies for Translation into Safer and More Effective Cancer Therapeutics. In Aromatherapy: Basic Mechanisms and Evidence-Based Clinical Use; Bagetta, G., Cosentino, M., Sakurada, T., Eds.; CRC Press: Boca Raton, FL, USA, 2016; p. 170. ISBN 978-1-4822-4663-6. [Google Scholar]
  48. Andrade-Ochoa, S.; Sánchez-Aldana, D.; Chacón-Vargas, K.F.; Rivera-Chavira, B.E.; Sánchez-Torres, L.E.; Camacho, A.D.; Nogueda-Torres, B.; Nevárez-Moorillón, G.V. Oviposition Deterrent and Larvicidal and Pupaecidal Activity of Seven Essential Oils and their Major Components against Culex quinquefasciatus Say (Diptera: Culicidae): Synergism-antagonism Effects. Insects 2018, 9, 25. [Google Scholar] [CrossRef] [PubMed]
  49. Nibret, E.; Wink, M. Trypanocidal and antileukaemic effects of the essential oils of Hagenia abyssinica, Leonotis ocymifolia, Moringa stenopetala, and their main individual constituents. Phytomedicine 2010, 17, 911–920. [Google Scholar] [CrossRef] [PubMed]
  50. Ngahang Kamte, S.L.; Ranjbarian, F.; Cianfaglione, K.; Sut, S.; Dall’Acqua, S.; Bruno, M.; Afshar, F.H.; Iannarelli, R.; Benelli, G.; Cappellacci, L.; et al. Identification of highly effective antitrypanosomal compounds in essential oils from the Apiaceae family. Ecotoxicol. Environ. Saf. 2018, 156, 154–165. [Google Scholar] [CrossRef] [PubMed]
  51. Sliwowski, J.K.; Caspi, E. An improved method of preparation of plates and sheets for thin-layer argentation chromatography. J. Steroid Biochem. 1977, 8, 42–49. [Google Scholar] [CrossRef]
  52. Sykes, M.L.; Avery, V.M. Development of an Alamar Blue™ Viability Assay in 384-Well Format for High Throughput Whole Cell Screening of Trypanosoma brucei brucei Bloodstream Form Strain 427. Am. J. Trop. Med. Hyg. 2009, 81, 665–674. [Google Scholar] [CrossRef] [PubMed]
  53. Ioset, L.-R.; Brun, R.; Wenzler, T.; Kaiser, M.; Yardley, V. Drug Screening for Kinetoplastids Diseases, a Training Manual for Screening in Neglected Diseases; The Pan-Asian Screening Network; DNDi: Geneva, Switzerland, 2009; pp. 20–21. [Google Scholar]
  54. Hirumi, H.; Himuri, K. Axenic culture of African trypanosome bloodstream forms. Parasitol. Today 1994, 10, 80–84. [Google Scholar] [CrossRef]
  55. Koch, A.; Basar, S.; Richter, R. TLC of Mono- and Sesquiterpenes. In Thin Layer Chromatography in Phytochemistry; Waksmundzka-Hajnos, M., Sherma, J., Kowalska, T., Eds.; CRC Press: Boca Raton, FL, USA, 2008; pp. 459–461. ISBN 978-1-4200-4677-9. [Google Scholar]
  56. Williams, C.M.; Mander, L.N. Chromatography with silver nitrate. Tetrahedron 2001, 57, 425–447. [Google Scholar] [CrossRef]
  57. Morris, L.J. Separations of lipids by silver ion chromatography. J. Lipid Res. 1966, 7, 717–732. [Google Scholar] [PubMed]
  58. Denyer, C.V.; Jackson, P.; Loakes, D.M.; Ellis, M.R.; Young, D.A.B. Isolation of antirhinoviral sesquiterpenes from ginger (Zingiber officinale). J. Nat. Prod. 1994, 57, 658–662. [Google Scholar] [CrossRef] [PubMed]
  59. Wang, Y.; Harrison, L.J.; Tan, B.C. Terpenoids from the liverwort Chandonanthus hirtellus. Tetrahedron 2009, 65, 4035–4043. [Google Scholar] [CrossRef]
  60. Fujiwaraj, M.; Yagi, N.; Miyazawa, M. Acetylcholinesterase inhibitory activity of volatile oil from Peltophorum dasyrachis Kurz ex Bakar (Yellow Batai) and bisabolane-type sesquiterpenoids. J. Agric. Food Chem. 2010, 58, 2824–2829. [Google Scholar] [CrossRef] [PubMed]
  61. Ragasa, C.Y.; Laguardia, M.A.; Rideout, J.A. Antimicrobial sesquiterpenoids and diarylheptanoid from Curcuma domestica. ACGC Chem. Res. Commun. 2005, 18, 21–24. [Google Scholar]
Sample Availability: Samples of essential oils are available from the authors.
Molecules 24 01158 g001
Figure 1. Viability percentages of Tbb bloodstream form treated with 25 nL/mL (in black) or 10 nL/mL (in white) of EO. Mean ± SD calculated in triplicate repeated twice and normalized according to negative control. The IC50 of the positive control (suramin) in those experiments was 21.53 ± 2.62 ng/mL.
Figure 1. Viability percentages of Tbb bloodstream form treated with 25 nL/mL (in black) or 10 nL/mL (in white) of EO. Mean ± SD calculated in triplicate repeated twice and normalized according to negative control. The IC50 of the positive control (suramin) in those experiments was 21.53 ± 2.62 ng/mL.
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Molecules 24 01158 g002
Figure 2. Chemical structures of five major compounds tested from C. longa EO.
Figure 2. Chemical structures of five major compounds tested from C. longa EO.
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Figure 3. Isolation of β-sesquiphellandrene, ar-curcumene, and curlone from C. longa EO.
Figure 3. Isolation of β-sesquiphellandrene, ar-curcumene, and curlone from C. longa EO.
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Table 1. Anti-trypanosomal activity, cytotoxicity, and selectivity indices of the 19 selected EOs.
Table 1. Anti-trypanosomal activity, cytotoxicity, and selectivity indices of the 19 selected EOs.
Plant Species (Studied Parts)Anti-Trypanosomal Activity (IC50 nL/mL)Cytotoxicity (IC50 nL/mL)
WI38SI J774SI
Amomum aromaticum (fruits)8.75 ± 1.2547.31 ± 0.305.422.68 ± 3.222.6
Artemisia annua (leaves)8.99 ± 1.1845.64 ± 1.025.138.16 ± 0.214.2
Cinnamomum cassia (stem barks)1.77 ± 0.1511.97 ± 0.936.88.97 ± 0.665.1
Clausena indica (leaves)13.22 ± 4.54>50.00>3.8>50.00>3.8
Curcuma longa (rhizomes)3.17 ± 0.7246.00 ± 0.3314.544.11 ± 3.1313.9
Curcuma zedoaria (rhizomes)2.51 ± 1.0846.64 ± 0.9518.626.81 ± 1.5910.7
Dysphania ambrosioides (aerial parts)2.86 ± 0.32>50.00>17.512.29 ± 2.924.3
Elsholtzia blanda (leaves)8.23 ± 1.03>50.00>6.1>50.00>6.1
Elsholtzia ciliata (leaves)4.26 ± 0.8648.46 ± 0.1211.413.21 ± 1.483.1
Elsholtzia communis (leaves)18.39 ± 3.32>50.00>2.740.68 ± 3.442.2
Hedychium coronarium (rhizomes)9.73 ± 1.43>50.00>5.130.00 ± 4.063.1
Kaempferia galangal (rhizomes)15.78 ± 3.29>50.00>3.2>50.00>3.2
Litsea cubeba (fruits)2.67 ± 1.12>50.00>18.7>50.00>18.7
Litsea cubeba (leaves)16.47 ± 1.24>50.00>3.0>50.00>3.0
Pluchea indica (leaves)21.29 ± 1.3827.47 ± 1.491.325.05 ± 5.561.2
Pogostemon cablin (leaves)4.07 ± 0.9827.17 ± 3.626.728.40 ± 1.817.0
Vitex trifolia (leaves)3.24 ± 0.7931.12 ± 2.839.626.64 ± 0.768.2
Zingiber officinale (rhizomes)3.10 ± 0.08>50.00>16.137.52 ± 0.0512.1
Zingiber zerumbet (rhizomes)6.23 ± 0.733.65 ± 0.340.62.78 ± 0.57 0.5
Suramin21.53 ± 2.62a
Camptothecin 34.99 ± 9.63 a 7.32 ± 1.29 a
IC50: Mean ± SD calculated in at least triplicate for anti-trypanosomal activity and duplicate for cytotoxicity; a concentration in ng/mL.
Table 2. Chemical composition of the four selected EOs.
Table 2. Chemical composition of the four selected EOs.
No.CompoundsRIRelative Percentage (%)Identification
L. cubebaC. zedoariaZ. officinaleC. longa
1α-Pinene m5360.740.112.29-MS, Co-GC, Ref.
2α-Thujene m5400.18---MS, Ref.
3Camphene m577-0.266.94-MS, Ref.
4β-Pinene m6210.860.770.160.09MS, Co-GC, Ref.
5Sabinene m6350.83-0.19-MS, Co-GC, Ref.
63-Carene m6650.36---MS
7α-Phellandrene m679--0.700.08MS, Co-
8Myrcene m6811.25-1.10tMS, Co-GC, Ref.
9α-Terpinene m6970.51--tMS, Co-GC, Ref.
10Limonene m7148.720.182.060.19MS, Co-GC, Ref.
11β-Phellandrene m7270.16-14.78-MS
12Eucalyptol m7271.371.611.793.15MS, Co-GC, Ref.
13γ-Terpinene m7610.52-ttMS, Co-GC, Ref.
14p-Cymeme m7830.10tttMS, Co-GC, Ref.
15Terpinolene m7980.31-0.261.70MS, Co-GC, Ref.
162-Heptanol844-0.140.15tMS
175-Hepten-2-one, 6-methyl-8540.35-t-MS, Co-GC
185-Heptenal, 2,6-dimethyl8670.68---MS
192-Nonanone903-0.430.15tMS
20(E)-2-Octenal940--t-MS
212-Octanol941-t--MS
22p-Cymenene m946---tMS
231-Octen-3-ol969-t--MS, Ref.
24δ-Elemene s980-0.30ttMS
25Cyclosativene s986--t-MS
26Citronellal m99343.10-0.300.14MS, Co-GC, Ref.
27α-Copaene s999--0.31-MS
28Decanone1006-t--MS
29Camphor m1020-4.18t-MS, Co-GC, Ref.
302-Nonanol1038-2.160.190.21MS
31Linalool m10635.600.220.48tMS, Co-GC, Ref.
32cis-α-Bergamotene s1065--t0.27MS
33Pulegol m10726.52---MS
34Isopulegol m108211.10---MS, Ref.
35trans-α-Bergamotene s1091---0.12MS, Ref.
36β-Elemene s1096-4.850.340.22MS
37β-Caryophyllene s1100-3.790.43tMS, Co-GC, Ref.
382-Undecanone1106--0.39tMS
39Terpinene-4-ol m11092.580.310.220.13MS, Co-GC, Ref.
40γ-Elemene s1142-0.32-0.09MS
41α-Himachalene s1153---tMS
42γ-Gurjunene s1160---tMS
43α-Humulene s1168-1.28-tMS, Co-GC, Ref.
44(E)-β-Farnesene s1174--0.260.61MS
45Neral m1186--3.16-MS, Co-GC
46α-Terpineol m12030.620.231.780.48MS, Co-GC, Ref.
47Borneol m1208--1.35-MS, Co-GC, Ref.
48Germacrene D s1206-1.99-tMS, Ref.
49α-Muurolene s1217--1.44-MS
50β-Selinene s1218-1.76--MS
51β-Chamigrene s1223-1.47--MS
52α-Zingiberene s1236--27.7125.38MS, Co-GC
53β-Bisabolene s1238--7.273.38MS, Ref.
54α-Cubebene s1248--0.23-MS
55(E,E)-α-Farnesene s1259--3.710.36MS
56Citronellol m12745.17---MS, Co-GC, Ref.
57β-Sesquiphellandrene s1279--8.0818.27MS
58ar-Curcumene s1280--2.715.22MS
59ζ-Elemene s1323-2.470.55-MS
60Geraniol m1351--0.450.25MS, Co-GC, Ref.
61Curzerene s1366-4.87--MS
62Epiglobulol s1468-0.57--MS
63(E)-Nerolidol s1536--0.32-MS, Co-GC, Ref.
64Elemol s1569--0.18-MS
65Ledene oxide s1583-0.25--MS
66Bisabolone s1595---1.08MS
67Spathulenol s16070.21---MS
68ar-Turmerol s1658---1.60MS
69α-Turmerone s1667---10.28MS
70Bisabolol s1670---0.87MS
718,9-Dehydro-9-formyl cycloisolongifolene s1703-29.31--MS
72Germacrone s1710-8.95-3.34MS
73Curlone s1723---5.15MS
74ar-Turmerone s1739---9.93MS, Co-GC
75Curdione s1792-13.52--MS
76Farnesol s1838--0.20-MS, Ref.
Total identified 91.8486.3092.6392.59
m: monoterpene; s: sesquiterpene; t: trace (peak area less than 0.05%); RI: the retention index was calculated using a homologous series of fatty acid methyl esters C5–C27; MS: mass spectra (matching coefficient > 700 compared with NIST database); Co-GC: co-injection with pure compound; Ref.: reference [15,16]; major compounds are in bold.
Table 3. Anti-trypanosomal activity and cytotoxicity of five pure compounds identified in C. longa EO.
Table 3. Anti-trypanosomal activity and cytotoxicity of five pure compounds identified in C. longa EO.
CompoundsAnti-Trypanosomal Activity (IC50 µg/mL)Cytotoxicity (IC50 µg/mL)
WI38SIJ774SI
α-Zingiberene6.91 ± 2.6028.50 ± 1.434.129.64 ± 2.544.3
β-Sesquiphellandrene9.89 ± 1.1819.11 ± 1.581.921.02 ± 2.722.1
ar-Curcumene13.38 ± 2.4623.15 ± 1.361.724.03 ± 2.641.8
Curlone1.38 ± 0.5243.64 ± 2.4531.725.06 ± 3.4718.2
ar-Turmerone28.83 ± 3.9343.39 ± 3.891.544.62 ± 1.411.6
Suramin21.53 ± 2.62 a
Camptothecin 34.99 ± 9.63 a 7.32 ± 1.29 a
IC50: Mean ± SD calculated in at least triplicate for anti-trypanosomal activity and duplicate for cytotoxicity; a concentration in ng/mL.

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Le, T.B.; Beaufay, C.; Nghiem, D.T.; Pham, T.A.; Mingeot-Leclercq, M.-P.; Quetin-Leclercq, J. Evaluation of the Anti-Trypanosomal Activity of Vietnamese Essential Oils, with Emphasis on Curcuma longa L. and Its Components. Molecules 2019, 24, 1158. https://doi.org/10.3390/molecules24061158

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Le TB, Beaufay C, Nghiem DT, Pham TA, Mingeot-Leclercq M-P, Quetin-Leclercq J. Evaluation of the Anti-Trypanosomal Activity of Vietnamese Essential Oils, with Emphasis on Curcuma longa L. and Its Components. Molecules. 2019; 24(6):1158. https://doi.org/10.3390/molecules24061158

Chicago/Turabian Style

Le, Thanh Binh, Claire Beaufay, Duc Trong Nghiem, Tuan Anh Pham, Marie-Paule Mingeot-Leclercq, and Joëlle Quetin-Leclercq. 2019. "Evaluation of the Anti-Trypanosomal Activity of Vietnamese Essential Oils, with Emphasis on Curcuma longa L. and Its Components" Molecules 24, no. 6: 1158. https://doi.org/10.3390/molecules24061158

APA Style

Le, T. B., Beaufay, C., Nghiem, D. T., Pham, T. A., Mingeot-Leclercq, M.-P., & Quetin-Leclercq, J. (2019). Evaluation of the Anti-Trypanosomal Activity of Vietnamese Essential Oils, with Emphasis on Curcuma longa L. and Its Components. Molecules, 24(6), 1158. https://doi.org/10.3390/molecules24061158

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