Anti-Oxidative and Anti-Aging Activities of Porcine By-Product Collagen Hydrolysates Produced by Commercial Proteases: Effect of Hydrolysis and Ultrafiltration
1
Department of Food Science and Biotechnology, Sejong University, 209 Neungdong-ro, Seoul 05006, Korea
2
Animal Resources Research Center, Konkuk University, 120 Neungdong-ro, Seoul 05029, Korea
3
Department of Agriculture, Food and Nutritional Science, University of Alberta, 116 St. and Ave., Edmonton, AB T6G 2P5, Canada
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(6), 1104; https://doi.org/10.3390/molecules24061104
Received: 30 January 2019 / Revised: 19 March 2019 / Accepted: 19 March 2019 / Published: 20 March 2019
(This article belongs to the Special Issue Extraction of Bioactive Molecules from Food By-Products and Their Utilization as Functional Ingredients)
To investigate methods for improving the processing of porcine waste, porcine skin was hydrolyzed using different commercially available proteases (Alcalase, Flavorzyme, Neutrase, Bromeline, Protamex, and Papain) under several optimal conditions. Following enzymatic hydrolysis, the collagen hydrolysates (CHs) were fractionated by molecular weight (3 kDa) via membrane ultrafiltration. The CHs were analyzed for physical properties (pH, protein recovery, free amino group content, molecular weight distribution, and amino composition) as well as for functional properties (antioxidant activities and anti-aging activities). Among the CHs, CHs hydrolyzed by Alcalase (CH-Alcalase) exhibited the highest degree of hydrolysis compared to other CHs. Both “CH-Alcalase” and “CH-Alcalase < 3 kDa” fractions showed a considerably high antioxidant activity and collagenase inhibition activity. Therefore, resulting bioactives have potential for development as antioxidants and anti-aging ingredients in the food, cosmetics, and pharmaceuticals, from animal by-products.
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Keywords:
hydrolysis; commercial proteases; collagen hydrolysate; antioxidant; anti-aging
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MDPI and ACS Style
Hong, G.-P.; Min, S.-G.; Jo, Y.-J. Anti-Oxidative and Anti-Aging Activities of Porcine By-Product Collagen Hydrolysates Produced by Commercial Proteases: Effect of Hydrolysis and Ultrafiltration. Molecules 2019, 24, 1104.
AMA Style
Hong G-P, Min S-G, Jo Y-J. Anti-Oxidative and Anti-Aging Activities of Porcine By-Product Collagen Hydrolysates Produced by Commercial Proteases: Effect of Hydrolysis and Ultrafiltration. Molecules. 2019; 24(6):1104.
Chicago/Turabian StyleHong, Geun-Pyo; Min, Sang-Gi; Jo, Yeon-Ji. 2019. "Anti-Oxidative and Anti-Aging Activities of Porcine By-Product Collagen Hydrolysates Produced by Commercial Proteases: Effect of Hydrolysis and Ultrafiltration" Molecules 24, no. 6: 1104.
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