Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.
Abstract
:1. Introduction
2. Results and Discussion
2.1. 5-HTP Is the Major Nitrogen-Containing Compound of G. simplicifolia
2.2. Griffonia simplicifolia Seeds Show a High Content of Unsaturated Fatty Acids
2.3. DNA Fingerprinting of Griffonia simplicifolia Using PCR–RFLP Analysis
3. Materials and Methods
3.1. Plant Material
3.2. Extraction of 5-HTP and Other N-Containing Compounds
3.3. Identification and Quantification of 5-HTP and Other N-Containing Compounds
3.4. Identification and Quantification of Fatty Acids
3.5. DNA Extraction, PCR Amplification, Subcloning, and Sequencing
3.6. PCR–RFLP Analysis and DNA Fingerprinting
3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the Griffonia simplicifolia extract are available from the authors. |
Compound | MW | [M + H]+ | m/z | mg g−1 d.wt. | SD | Relative Percentage | ||
---|---|---|---|---|---|---|---|---|
1H-indole-3-carboxylic acid | 161.0 | 162.0 | 143.8 | 115.9 | 0.08 | 0.006 | 0.04 | |
5-hydroxytryptamine | 176.0 | 177.0 | 158.8 | 135.8 | 117.0 | 1.15 | 0.066 | 0.64 |
5-hydroxy-3-(2-hydroxyethyl)indole | 177.0 | 178.0 | 159.8 | 132.9 | 115.0 | 0.27 | 0.006 | 0.15 |
5-hydroxy-l-tryptophan (5-HTP) | 220.0 | 221.0 | 204.0 | 161.9 | 156.48 | 8.320 | 86.48 | |
3-carboxy-6-hydroxy-β-carboline | 228.0 | 229.0 | 210.0 | 183.0 | 101.1 | 2.69 | 0.120 | 1.49 |
hyrtioerectine B | 246.0 | 247.0 | 229.9 | 203.9 | 174.0 | 6.75 | 0.320 | 3.73 |
griffonine | 329.0 | 330.0 | 167.9 | 0.75 | 0.030 | 0.41 | ||
hyrtiosulawesine | 343.0 | 344.0 | 228.9 | 200.9 | 182.9 | 0.9 | 0.030 | 0.50 |
1,1′-ethylidenebis(l-tryptophan) | 434.0 | 435.0 | 231.0 | n.d. | n.d. | n.d. | ||
3-(phenylamino)alanine | 180.0 | 181.0 | 106.0 | 88.0 | 70.0 | n.d. | n.d. | n.d. |
4,5-tryptophan-dione | 234.0 | 235.0 | 217.0 | 162.0 | 181.0 | 11.87 | 0.92 | 6.56 |
TOTAL | 181.10 | 16.10 | 100.00 |
Fatty Acid | Kovat’s Index | mg/g | SD | Relative Percentage |
---|---|---|---|---|
palmitic acid | 1942 | 18.22 | 0.93 | 11.06% |
linoleic acid | 2095 | 91.24 | 5.74 | 55.38% |
oleic acid | 2113 | 20.24 | 1.13 | 12.28% |
stearic acid | 2187 | 32.12 | 2.25 | 19.50% |
arachidic acid | 2359 | 10.39 | 0.65 | 6.31% |
behenic acid | 2400 | 4.65 | 0.32 | 2.82% |
TOTAL | 164.76 | 11.09 | 100.00% |
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Vigliante, I.; Mannino, G.; Maffei, M.E. Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill. Molecules 2019, 24, 1032. https://doi.org/10.3390/molecules24061032
Vigliante I, Mannino G, Maffei ME. Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill. Molecules. 2019; 24(6):1032. https://doi.org/10.3390/molecules24061032
Chicago/Turabian StyleVigliante, Ivano, Giuseppe Mannino, and Massimo E. Maffei. 2019. "Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill." Molecules 24, no. 6: 1032. https://doi.org/10.3390/molecules24061032