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Molecules 2019, 24(6), 1025; https://doi.org/10.3390/molecules24061025

Polyphenols Content, Antioxidant Activity, and Cytotoxicity Assessment of Taraxacum officinale Extracts Prepared through the Micelle-Mediated Extraction Method

1
Department of Chemistry and Food Toxicology, Faculty of Biology and Agriculture, University of Rzeszow, Ćwiklińskiej 1a, 35-601 Rzeszów, Poland
2
Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
3
Department of Biotechnology and Bioinformatics, University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland
*
Author to whom correspondence should be addressed.
Received: 1 February 2019 / Revised: 4 March 2019 / Accepted: 12 March 2019 / Published: 14 March 2019
(This article belongs to the Section Chemical Biology)
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Abstract

This experiment was conducted with extracts prepared from dandelion (Taraxacum officinale F. H. Wigg) leaves and flowers, using the micelle-mediated extraction method, with the surface active compound Triton X-100 and water–acetone as the extraction solvents. Extracts were, first, examined for the content of total phenols and the antioxidant capacity. All extracts showed good anti-radical properties, especially for leaves, in comparison to the flower samples. Flavonoids (mainly luteolin derivatives) and phenolic acids, predominated among the determined polyphenols. Quantitative analyses indicated acetone extract to be the richest in phenols (up to 0.535 mg/mL), in the case of dandelion leaves, and Triton X-100 extract in the case of flowers (0.385 mg/mL). Extracts were also evaluated for cytotoxicity to the model cell line (epithelial rabbit kidney cells RK13), using the colorimetric 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test and the real-time cell analysis method ((RTCA); xCELLigence system). The obtained results indicated that surfactants, especially non-ionic ones, can be effectively used as modifiers in the aqueous extraction of phenolic compounds from plant materials. An advantage over the traditional organic solvents is their non-flammability. Furthermore, surfactants might also be used at low concentrations. Studies on cell lines, however, indicated the cytotoxic effect of this type of compound, even in the trace amounts present in the extracts. View Full-Text
Keywords: antioxidants; cytotoxicity; micelle-mediated extraction; xCELLigence system; UHPLC-MS/MS antioxidants; cytotoxicity; micelle-mediated extraction; xCELLigence system; UHPLC-MS/MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Miłek, M.; Marcinčáková, D.; Legáth, J. Polyphenols Content, Antioxidant Activity, and Cytotoxicity Assessment of Taraxacum officinale Extracts Prepared through the Micelle-Mediated Extraction Method. Molecules 2019, 24, 1025.

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