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Molecules 2019, 24(4), 661; https://doi.org/10.3390/molecules24040661

Short-Term Neonatal Oral Administration of Oleanolic Acid Protects against Fructose-Induced Oxidative Stress in the Skeletal Muscles of Suckling Rats

1
Department of Human Anatomy and Physiology, Faculty of Health Sciences, University of Johannesburg, Doornfontein, Johannesburg 2028, South Africa
2
Department of Biochemistry, Faculty of Natural Sciences & Agriculture, North West University, Mafikeng, Mmabatho 2735, South Africa
3
Department of Biochemistry, Faculty of Science, Adeleke University, P.M.B. 250, Ede 232, Osun State, Nigeria
4
Agricultural Research Council, Vegetable and Ornamental Plants (VOP), Private Bag X293, Pretoria 0001, South Africa
5
School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Johannesburg 2193, South Africa
*
Author to whom correspondence should be addressed.
Academic Editors: Natália Martins and Gertjan Van Dijk
Received: 29 November 2018 / Revised: 22 January 2019 / Accepted: 28 January 2019 / Published: 13 February 2019
(This article belongs to the Special Issue Bioactive Plant Compounds for Sustainable Health)
Full-Text   |   PDF [760 KB, uploaded 13 February 2019]   |  

Abstract

Nutritional manipulations in the neonatal period are associated with the development of negative or positive health outcomes later in life. Excessive fructose consumption has been attributed to the increase in the global prevalence of metabolic syndrome (MetS) and the development of oxidative stress. Oleanolic acid (OA) has anti-diabetic and anti-obesity effects. We investigated the protective potential of orally administering OA in the neonatal period, to prevent fructose-induced oxidative stress, adverse health outcomes and maturation of the gastrointestinal tract (GIT) in suckling rats. Seven-day old Sprague-Dawley rats (N = 30) were gavaged daily with 10 mL/kg of: distilled water (DW), oleanolic acid (OA; 60 mg/kg), high fructose solution (HF; 20% w/v), or OAHF for 7 days. On day 14, tissue samples were collected to determine clinical health profiles, hepatic lipid content, and activity of anti-oxidant enzymes. Furthermore, biomarkers of oxidative stress and anti-oxidant capacity in the skeletal muscles were assessed. The gastrointestinal tract (GIT) morphometry was measured. Rats in all groups grew over the 7-day treatment period. There were no significant differences in the terminal body masses, GIT morphometry, surrogate markers of general health, liver lipid content across all treatment groups (p < 0.05). Neonatal fructose administration decreased the activity of catalase, depleted GSH and increased lipid peroxidation. However, the level of GSH and catalase activity were improved by neonatal OA treatment. Short-term oral OA administration during the critical developmental period protects against fructose-induced oxidative stress without adverse effects on health outcomes associated with MetS or precocious development of the GIT in suckling male and female rats. View Full-Text
Keywords: oleanolic acid; oxidative damage; neonatal programming; metabolic syndrome; oxidative stress; anti-oxidant enzymes; high fructose oleanolic acid; oxidative damage; neonatal programming; metabolic syndrome; oxidative stress; anti-oxidant enzymes; high fructose
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Nyakudya, T.T.; Isaiah, S.; Ayeleso, A.; Ndhlala, A.R.; Mukwevho, E.; Erlwanger, K.H. Short-Term Neonatal Oral Administration of Oleanolic Acid Protects against Fructose-Induced Oxidative Stress in the Skeletal Muscles of Suckling Rats. Molecules 2019, 24, 661.

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