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Open AccessArticle

In-Vitro Antioxidant, Hypoglycemic Activity, and Identification of Bioactive Compounds in Phenol-Rich Extract from the Marine Red Algae Gracilaria edulis (Gmelin) Silva

1
Department of Zoology, Faculty of Applied Sciences (Center for Instrumentation Facility & Center for Biotechnology), University of Sri Jayewardenepura, Nugegoda 10250, Sri Lanka
2
National Science and Technology Commission, Dudley Senanayake Mawatha, Colombo 8 00800, Sri Lanka
3
Industrial Technology Institute, Halbarawa Gardens, Malabe 10115, Sri Lanka
*
Author to whom correspondence should be addressed.
Academic Editor: Lorenzo Di Cesare Mannelli
Molecules 2019, 24(20), 3708; https://doi.org/10.3390/molecules24203708
Received: 4 July 2019 / Revised: 22 September 2019 / Accepted: 3 October 2019 / Published: 15 October 2019
(This article belongs to the Special Issue Plant Extracts: Biological and Pharmacological Activity)
Obesity and diabetes are major metabolic disorders which are prevalent worldwide. Algae has played an important role in managing these disorders. In this study, Gracilaria edulis, a marine red algae, was investigated for antioxidant and hypoglycemic potential using in vitro models. De-polysaccharide methanol extract of G. edulis was sequentially partitioned with hexane, chloroform, ethyl acetate, and antioxidants, and hypoglycemic potentials were evaluated using multiple methods. High antioxidant potential was observed in the ethyl acetate fraction in terms of ferric reducing antioxidant power, iron chelating, and DPPH and ABTS radical scavenging activities, while the crude methanol extract exhibited potent oxygen radical-absorbance capacity. Potent α-amylase inhibitory activity was observed in the ethyl acetate fraction, while the ethyl acetate fraction was effective against α-glucosidase inhibition. Glucose diffusion was inhibited by the ethyl acetate fraction at 180 min, and the highest antiglycation activity was observed in both chloroform and ethyl acetate fractions. Additionally, gas chromatography-mass spectrometry analysis of the ethyl acetate fraction revealed the presence of several potent anti-diabetic compounds. In conclusion, G. edulis exhibited promising antidiabetic potential via multiple mechanisms. The ethyl acetate fraction exhibited the strongest hypoglycemic and antiglycation potential among the four fractions, and hence the isolation of active compounds is required to develop leads for new drugs to treat diabetes. View Full-Text
Keywords: Gracillaria edulis; methanol extract; fractionation; α-amylase; α-glucosidase; antiglycation; glucose diffusion Gracillaria edulis; methanol extract; fractionation; α-amylase; α-glucosidase; antiglycation; glucose diffusion
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MDPI and ACS Style

Gunathilaka, T.L.; Samarakoon, K.W.; Ranasinghe, P.; Peiris, L.D.C. In-Vitro Antioxidant, Hypoglycemic Activity, and Identification of Bioactive Compounds in Phenol-Rich Extract from the Marine Red Algae Gracilaria edulis (Gmelin) Silva. Molecules 2019, 24, 3708.

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