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Open AccessArticle

Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa

1
Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, Russia
2
Department of Morphology and General Pathology, Siberian State Medical University, Tomsk 634050, Russia
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Department of Pharmaceutical Analysis, Siberian State Medical University, Tomsk 634050, Russia
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Basic Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, Russia
5
Department of Microbiology and Virology, Siberian State Medical University, Tomsk 634050, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Molecules 2019, 24(20), 3680; https://doi.org/10.3390/molecules24203680
Received: 6 September 2019 / Revised: 25 September 2019 / Accepted: 11 October 2019 / Published: 12 October 2019
(This article belongs to the Collection Herbal Medicine Research)
The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC>F-SC) at doses of 10–50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.
Keywords: Saussurea controversa DC (Asteraceae); polysaccharides; flavonoids; human adipose-derived multipotent mesenchymal stromal cells; cytotoxicity; alizarin red staining; Staphylococcus aureus growth Saussurea controversa DC (Asteraceae); polysaccharides; flavonoids; human adipose-derived multipotent mesenchymal stromal cells; cytotoxicity; alizarin red staining; Staphylococcus aureus growth
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Khlusov, I.; Avdeeva, E.; Shupletsova, V.; Khaziakhmatova, O.; Litvinova, L.; Porokhova, E.; Reshetov, Y.; Zvereva, I.; Mushtovatova, L.; Karpova, M.; Guryev, A.; Sukhodolo, I.; Belousov, M. Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa. Molecules 2019, 24, 3680.

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