Next Article in Journal
Phyto-Nanocatalysts: Green Synthesis, Characterization, and Applications
Next Article in Special Issue
A Complete Study of Farrerol Metabolites Produced In Vivo and In Vitro
Previous Article in Journal
Exploring the Synergy Between HPTLC and HPLC-DAD for the Investigation of Wine-Making By-Products
Previous Article in Special Issue
Determination of Urinary Pterins by Capillary Electrophoresis Coupled with LED-Induced Fluorescence Detector
Open AccessArticle

Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from Thalictrum foetidum, Berberis sp. and Chelidonium majus Extracts

1
Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
2
Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
3
Department of Pharmacology, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
4
Botanical Garden of Maria Curie-Skłodowska University in Lublin, 20-819 Lublin, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Marcello Locatelli
Molecules 2019, 24(19), 3417; https://doi.org/10.3390/molecules24193417
Received: 20 July 2019 / Revised: 16 September 2019 / Accepted: 18 September 2019 / Published: 20 September 2019
Background: Plants are an important origin of natural substances that the raw material for various pharmaceutical and therapeutic applications due to the presence of phytochemicals, such as alkaloids. Alkaloids, which are found in different plant species, possess numerous biological activities. Some alkaloids have strong cytotoxic effects on various cancer cells. The search for new drugs to treat various cancers is one of the most important challenges of modern scientific research. Objective: This study aimed to investigate of cytotoxic activity of extracts that were obtained from Chelidonium Majus; Berberis sp.; Thalictrum foetidum containing various alkaloids on selected cancer cell lines. The aim was also the quantification of selected alkaloids in the investigated extracts by HPLC. Methods: The analysis of alkaloids contents were performed while using HPLC in reversed phase (RP) mode using Polar RP column and mobile phase containing acetonitrile, water, and ionic liquid. The cytotoxic effect of the tested plant extracts and respective alkaloids’ standards were examined while using human pharyngeal squamous carcinoma cells (FaDu), human tongue squamous carcinoma cells (SCC-25), human breast adenocarcinoma cell line (MCF-7), and human triple-negative breast adenocarcinoma cell line (MDA-MB-231). Conclusion: All of the investigated plant extracts possess cytotoxic activity against cancer cell lines: FaDu, SCC-25, MCF-7, and MDA-MB-231. The highest cytotoxic activity against FaDu and MDA-MB-231 cells was observed for Chelidonium majus root extract, while the highest cytotoxic activity against SCC-25 and MCF-7 cells was estimated for the Thalictrum foetidum root extract. There obtained significant differences in the cytotoxic activity of extracts that were obtained from the roots and herbs of Chelidonium majus and Thalictrum foetidum. Based on these results, investigated plant extracts can be recommended for further investigations of anticancer activity. View Full-Text
Keywords: isoquinoline alkaloids; HPLC-DAD; Chelidonium majus; Berberis sp.; Thalictrum foetidum; cytotoxic activity; etoposide isoquinoline alkaloids; HPLC-DAD; Chelidonium majus; Berberis sp.; Thalictrum foetidum; cytotoxic activity; etoposide
Show Figures

Graphical abstract

MDPI and ACS Style

Petruczynik, A.; Tuzimski, T.; Plech, T.; Misiurek, J.; Szalast, K.; Szymczak, G. Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from Thalictrum foetidum, Berberis sp. and Chelidonium majus Extracts. Molecules 2019, 24, 3417.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop