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Purification, Characterization, and Application for Preparation of Antioxidant Peptides of Extracellular Protease from Pseudoalteromonas sp. H2

School of Life Sciences, Central South University, Changsha 410013, China
Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China
Department of Biochemistry, Changzhi Medical College, Changzhi 046000, China
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China
Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China
Authors to whom correspondence should be addressed.
Molecules 2019, 24(18), 3373;
Received: 13 August 2019 / Revised: 6 September 2019 / Accepted: 14 September 2019 / Published: 16 September 2019
(This article belongs to the Section Chemical Biology)
The study reported on the isolation of a metalloprotease named EH2 from Pseudoalteromonas sp. H2. EH2 maintained more than 80% activity over a wide pH range of 5–10, and the stability was also nearly independent of pH. Over 65% activity was detected at a wide temperature range of 20–70 °C. The high stability of the protease in the presence of different surfactants and oxidizing agents was also observed. Moreover, we also investigated the antioxidant activities of the hydrolysates generated from porcine and salmon skin collagen by EH2. The results showed that salmon skin collagen hydrolysates demonstrated higher DPPH (1,1-diphenyl-2-picrylhydrazyl) (42.88% ± 1.85) and hydroxyl radical (61.83% ± 3.05) scavenging activity than porcine skin collagen. For oxygen radical absorbance capacity, the hydrolysates from porcine skin collagen had higher efficiency (7.72 ± 0.13 μmol·TE/μmol). Even 1 nM mixed peptides could effectively reduce the levels of intracellular reactive oxygen species. The two types of substrates exerted the best antioxidant activity when hydrolyzed for 3 h. The hydrolysis time and type of substrate exerted important effects on the antioxidant properties of hydrolysates. The hydrolyzed peptides from meat collagens by proteases have good antioxidant activity, which may have implications for the potential application of marine proteases in the biocatalysis industry. View Full-Text
Keywords: Pseudoalteromonas; protease; hydrolysis; collagen; antioxidant peptides Pseudoalteromonas; protease; hydrolysis; collagen; antioxidant peptides
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Liu, D.; Huang, J.; Wu, C.; Liu, C.; Huang, R.; Wang, W.; Yin, T.; Yan, X.; He, H.; Chen, L. Purification, Characterization, and Application for Preparation of Antioxidant Peptides of Extracellular Protease from Pseudoalteromonas sp. H2. Molecules 2019, 24, 3373.

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