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Open AccessArticle

Identification of Potential Auxin-Responsive Small Signaling Peptides through a Peptidomics Approach in Arabidopsis thaliana

1
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
2
School of Life Sciences, Tsinghua University, Beijing 100084, China
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(17), 3146; https://doi.org/10.3390/molecules24173146
Received: 29 June 2019 / Revised: 14 August 2019 / Accepted: 28 August 2019 / Published: 29 August 2019
Small signaling peptides (SSPs) are a class of short peptides playing critical roles in plant growth and development. SSPs are also involved in the phytohormone signaling pathway. However, identification of mature SSPs is still a technical challenge because of their extremely low concentrations in plant tissue and complicated interference by many other metabolites. Here, we report an optimized protocol to extract SSPs based on protoplast extraction and to analyze SSPs based on tandem mass spectrometry peptidomics. Using plant protoplasts as the material, soluble peptides were directly extracted into phosphate buffer. The interference of non-signaling peptides was significantly decreased. Moreover, we applied the protocol to identify potential SSPs in auxin treated wild type and auxin biosynthesis defective mutant yuc2yuc6. Over 100 potential SSPs showed a response to auxin in Arabidopsis thaliana. View Full-Text
Keywords: small signaling peptides; plant protoplast; peptidomics; auxin small signaling peptides; plant protoplast; peptidomics; auxin
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MDPI and ACS Style

Luo, W.; Xiao, Y.; Liang, Q.; Su, Y.; Xiao, L. Identification of Potential Auxin-Responsive Small Signaling Peptides through a Peptidomics Approach in Arabidopsis thaliana. Molecules 2019, 24, 3146.

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