Next Article in Journal
A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk
Next Article in Special Issue
Appraisal of Comparative Therapeutic Potential of Undoped and Nitrogen-Doped Titanium Dioxide Nanoparticles
Previous Article in Journal
Characterization of a Cis-Prenyltransferase from Lilium longiflorum Anther
Previous Article in Special Issue
The Stimulatory Effects of Nanochitin Whisker on Carbon and Nitrogen Metabolism and on the Enhancement of Grain Yield and Crude Protein of Winter Wheat
Open AccessArticle

Centrifugation Conditions in the L-PRP Preparation Affect Soluble Factors Release and Mesenchymal Stem Cell Proliferation in Fibrin Nanofibers

1
Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas, SP 13083-852, Brazil
2
Hematology & Hemotherapy Center, Umbilical Cord Blood Bank, University of Campinas, Campinas, SP 13083-878, Brazil
3
Bone and Cartilage Institute, Indaiatuba, SP 13334-170, Brazil
*
Author to whom correspondence should be addressed.
Academic Editors: Marco Rossi, Daniele Passeri and Francesca A. Scaramuzzo
Molecules 2019, 24(15), 2729; https://doi.org/10.3390/molecules24152729
Received: 10 June 2019 / Revised: 15 July 2019 / Accepted: 19 July 2019 / Published: 27 July 2019
Leukocyte and platelet-rich plasma (L-PRP) is an autologous product that when activated forms fibrin nanofibers, which are useful in regenerative medicine. As an important part of the preparation of L-PRP, the centrifugation parameters may affect the release of soluble factors that modulate the behavior of the cells in the nanofibers. In this study, we evaluated the influences of four different centrifugation conditions on the concentration of platelets and leukocytes in L-PRP and on the anabolic/catabolic balance of the nanofiber microenvironment. Human adipose-derived mesenchymal stem cells (h-AdMSCs) were seeded in the nanofibers, and their viability and growth were evaluated. L-PRPs prepared at 100× g and 100 + 400× g released higher levels of transforming growth factor (TGF)-β1 and platelet-derived growth factor (PDGF)-BB due to the increased platelet concentration, while inflammatory cytokines interleukin (IL)-8 and tumor necrosis factor (TNF)-α were more significantly released from L-PRPs prepared via two centrifugation steps (100 + 400× g and 800 + 400× g) due to the increased concentration of leukocytes. Our results showed that with the exception of nanofibers formed from L-PRP prepared at 800 + 400× g, all other microenvironments were favorable for h-AdMSC proliferation. Here, we present a reproducible protocol for the standardization of L-PRP and fibrin nanofibers useful in clinical practices with known platelet/leukocyte ratios and in vitro evaluations that may predict in vivo results. View Full-Text
Keywords: platelet; leukocyte; L-PRP; centrifugation; fibrin; nanofiber; growth factor; cytokine; mesenchymal stem cells platelet; leukocyte; L-PRP; centrifugation; fibrin; nanofiber; growth factor; cytokine; mesenchymal stem cells
Show Figures

Graphical abstract

MDPI and ACS Style

de Melo, B.A.G.; Luzo, Â.C.M.; Lana, J.F.S.D.; Santana, M.H.A. Centrifugation Conditions in the L-PRP Preparation Affect Soluble Factors Release and Mesenchymal Stem Cell Proliferation in Fibrin Nanofibers. Molecules 2019, 24, 2729.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop