Next Article in Journal
Centrifugation Conditions in the L-PRP Preparation Affect Soluble Factors Release and Mesenchymal Stem Cell Proliferation in Fibrin Nanofibers
Next Article in Special Issue
Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
Previous Article in Journal
Erianin Inhibits Proliferation and Induces Apoptosis of HaCaT Cells via ROS-Mediated JNK/c-Jun and AKT/mTOR Signaling Pathways
Previous Article in Special Issue
Oxidosqualene Cyclase Knock-Down in Latex of Taraxacum koksaghyz Reduces Triterpenes in Roots and Separated Natural Rubber
Open AccessArticle

Characterization of a Cis-Prenyltransferase from Lilium longiflorum Anther

1
Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan
2
Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan
3
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editors: Ewa Swiezewska, Liliana Surmacz and Bernhard Loll
Molecules 2019, 24(15), 2728; https://doi.org/10.3390/molecules24152728
Received: 27 June 2019 / Revised: 19 July 2019 / Accepted: 25 July 2019 / Published: 26 July 2019
(This article belongs to the Special Issue Plant Isoprenoids)
A group of prenyltransferases catalyze chain elongation of farnesyl diphosphate (FPP) to designated lengths via consecutive condensation reactions with specific numbers of isopentenyl diphosphate (IPP). cis-Prenyltransferases, which catalyze cis-double bond formation during IPP condensation, usually synthesize long-chain products as lipid carriers to mediate peptidoglycan biosynthesis in prokaryotes and protein glycosylation in eukaryotes. Unlike only one or two cis-prenyltransferases in bacteria, yeast, and animals, plants have several cis-prenyltransferases and their functions are less understood. As reported here, a cis-prenyltransferase from Lilium longiflorum anther, named LLA66, was expressed in Saccharomyces cerevisiae and characterized to produce C40/C45 products without the capability to restore the growth defect from Rer2-deletion, although it was phylogenetically categorized as a long-chain enzyme. Our studies suggest that evolutional mutations may occur in the plant cis-prenyltransferase to convert it into a shorter-chain enzyme. View Full-Text
Keywords: isoprenoid; prenyltransferase; polyisoprenoid; dolichol; glycoprotein isoprenoid; prenyltransferase; polyisoprenoid; dolichol; glycoprotein
Show Figures

Figure 1

MDPI and ACS Style

Yao, J.-Y.; Teng, K.-H.; Liu, M.-C.; Wang, C.-S.; Liang, P.-H. Characterization of a Cis-Prenyltransferase from Lilium longiflorum Anther. Molecules 2019, 24, 2728.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop