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Functional Glyco-Nanogels for Multivalent Interaction with Lectins

1
Fraunhofer Institute for Applied Polymer Research IAP, Biofunctionalized Materials and (Glyco)Biotechnology, Geiselbergstr. 69, 14476 Potsdam, Germany
2
Fraunhofer Institute for Applied Polymer Research IAP, Biomaterials and Healthcare, Geiselbergstr. 69, 14476 Potsdam, Germany
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Author to whom correspondence should be addressed.
Academic Editor: Lothar Elling
Molecules 2019, 24(10), 1865; https://doi.org/10.3390/molecules24101865
Received: 9 April 2019 / Revised: 3 May 2019 / Accepted: 14 May 2019 / Published: 15 May 2019
(This article belongs to the Special Issue Synthesis and Biological Applications of Glycoconjugates Ⅱ)
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Abstract

Interactions between glycans and proteins have tremendous impact in biomolecular interactions. They are important for cell–cell interactions, proliferation and much more. Here, we emphasize the glycan-mediated interactions between pathogens and host cells. Pseudomonas aeruginosa, responsible for a huge number of nosocomial infections, is especially the focus when it comes to glycan-derivatives as pathoblockers. We present a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose. The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels. The influence of reaction parameters like crosslinker type or stabilizer amount was investigated. The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding. Due to multivalent presentation of glycans in the gel, the inhibition was clearly stronger than with unmodified saccharides, which was compared after determination of the glycan loading. First studies with Pseudomonas aeruginosa revealed a surprising influence on the secretion of virulence factors. Functional glycogels may be in the future potent alternatives or adjuvants for antibiotic treatment of infections based on glycan interactions between host and pathogen. View Full-Text
Keywords: nanogels; glycans; lectins; multivalency; glycogels; glycopolymers; precipitation polymerization nanogels; glycans; lectins; multivalency; glycogels; glycopolymers; precipitation polymerization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Tang, J.S.J.; Rosencrantz, S.; Tepper, L.; Chea, S.; Klöpzig, S.; Krüger-Genge, A.; Storsberg, J.; Rosencrantz, R.R. Functional Glyco-Nanogels for Multivalent Interaction with Lectins. Molecules 2019, 24, 1865.

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