The Effects of Berberine on the Gut Microbiota in Apc min/+ Mice Fed with a High Fat Diet
1
State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 210009, China
2
Department of Pathology, School of Medicine, Zhejiang University, Hangzhou 310058, China
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(9), 2298; https://doi.org/10.3390/molecules23092298
Received: 28 July 2018
/
Revised: 19 August 2018
/
Accepted: 6 September 2018
/
Published: 8 September 2018
(This article belongs to the Special Issue Natural Bioactives in Anti-Obesity Therapy)
Background: Berberine (BBR) has been extensively reported to inhibit colorectal cancer (CRC) development, though its bioavailability is poor. Nowadays, an increasing number of studies have shown that BBR significantly accumulates in the intestines and could regulate gut microbiota in obesity. The purpose of this study was to further explore the effects of BBR on gut microbiota in Apc min/+ mice receiving a high fat diet (HFD). Methods: Apc min/+ mice received either HFD alone or HFD and BBR for 12 weeks. The intestinal tissues were collected to evaluate the efficiency of BBR on neoplasm development by hematoxylin and eosin staining. Meanwhile, immunohistochemistry was conducted to investigate the effects of BBR on cyclin D1 and β-catenin in colon tissues. Fecal samples were subjected to 16S rRNA sequencing. Results: BBR significantly reduced intestinal tumor development and altered the structure of gut microbiota in Apc min/+ mice fed with an HFD. At the phylum level, it was able to significantly inhibit the increase in Verrucomicrobia. At the genus level, it was able to suppress Akkermansia and elevate some short chain fat acid (SCFA)-producing bacteria. Conclusions: BBR significantly alleviated the development of CRC in Apc min/+ mice fed with HFD and restored the enteric microbiome community.
View Full-Text
Keywords:
berberine; Apc min/+ mice; gut microbiota; high fat diet
▼
Show Figures
Graphical abstract
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Supplementary File 1:
ZIP-Document (ZIP, 600 KiB)
MDPI and ACS Style
Wang, H.; Guan, L.; Li, J.; Lai, M.; Wen, X. The Effects of Berberine on the Gut Microbiota in Apc min/+ Mice Fed with a High Fat Diet. Molecules 2018, 23, 2298. https://doi.org/10.3390/molecules23092298
AMA Style
Wang H, Guan L, Li J, Lai M, Wen X. The Effects of Berberine on the Gut Microbiota in Apc min/+ Mice Fed with a High Fat Diet. Molecules. 2018; 23(9):2298. https://doi.org/10.3390/molecules23092298
Chicago/Turabian StyleWang, Huan, Lingnan Guan, Jing Li, Maode Lai, and Xiaodong Wen. 2018. "The Effects of Berberine on the Gut Microbiota in Apc min/+ Mice Fed with a High Fat Diet" Molecules 23, no. 9: 2298. https://doi.org/10.3390/molecules23092298
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
