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Open AccessArticle

Protective Effect of Cyclically Pressurized Solid–Liquid Extraction Polyphenols from Cagnulari Grape Pomace on Oxidative Endothelial Cell Death

1
Department of Biomedical Sciences, School of Medicine, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
2
Porto Conte Ricerche S.r.l, Tramariglio, Alghero, 07041 Sassari, Italy
3
Department of Biomedical Sciences, College of Health Sciences, Qatar University, P.O. Box 2713 Doha, Qatar
4
Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, P.O. Box 2713 Doha, Qatar
5
Biomedical Research Center, Qatar University, P.O. Box 2713 Doha, Qatar
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2018, 23(9), 2105; https://doi.org/10.3390/molecules23092105
Received: 9 July 2018 / Revised: 17 August 2018 / Accepted: 17 August 2018 / Published: 21 August 2018
(This article belongs to the Special Issue Extractable and Non-Extractable Antioxidants)
The aim of this work is the evaluation of a green extraction technology to exploit winery waste byproducts. Specifically, a solid–liquid extraction technology (Naviglio Extractor®) was used to obtain polyphenolic antioxidants from the Cagnulari grape marc. The extract was then chemically characterized by spectrophotometric analysis, high-performance liquid chromatography, and mass spectrometry, revealing a total polyphenol content of 4.00 g/L ± 0.05, and the presence of anthocyanins, one of the most representative groups among the total polyphenols in grapes. To investigate potential biological activities of the extract, its ability to counteract hydrogen peroxide-induced oxidative stress and cell death was assessed in primary human endothelial cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, used to assess potential extract cytotoxicity, failed to show any deleterious effect on cultured cells. Fluorescence measurements, attained with the intracellular reactive oxygen species (ROS) probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA), revealed a strong antioxidant potential of the marc extract on the used cells, as indicated by the inhibition of the hydrogen peroxide-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate the Naviglio extraction, as a green technology process, can be used to exploit wine waste to obtain antioxidants which can be used to produce enriched foods and nutraceuticals high in antioxidants. View Full-Text
Keywords: Cagnulari marc; Naviglio Extractor®; green extraction; endothelial cell; oxidative stress; polyphenols Cagnulari marc; Naviglio Extractor®; green extraction; endothelial cell; oxidative stress; polyphenols
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Posadino, A.M.; Biosa, G.; Zayed, H.; Abou-Saleh, H.; Cossu, A.; Nasrallah, G.K.; Giordo, R.; Pagnozzi, D.; Porcu, M.C.; Pretti, L.; Pintus, G. Protective Effect of Cyclically Pressurized Solid–Liquid Extraction Polyphenols from Cagnulari Grape Pomace on Oxidative Endothelial Cell Death. Molecules 2018, 23, 2105.

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