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Molecules 2018, 23(7), 1652; https://doi.org/10.3390/molecules23071652

Cytotoxicity-Guided Isolation of Two New Phenolic Derivatives from Dryopteris fragrans (L.) Schott

1
College of Life Science, Northeast Agricultural University, Harbin 150030, China
2
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Pinarosa Avato
Received: 29 May 2018 / Revised: 21 June 2018 / Accepted: 1 July 2018 / Published: 6 July 2018
(This article belongs to the Special Issue Natural Products and Drug Discovery)
Full-Text   |   PDF [2065 KB, uploaded 6 July 2018]   |  

Abstract

Dryopteris fragrans is a valuable medicinal plant resource with extensive biological activities including anti-cancer, anti-oxidation, and anti-inflammation activities. This work aims to study further the cytotoxic constituents from Dryopteris fragrans. In this work, two new phenolic derivatives known as dryofragone (1) and dryofracoumarin B (2) with six known compounds (38) were isolated from the petroleum ether fraction of the methanol extract of the aerial parts of Dryopteris fragrans (L.) Schott by two round cytotoxicity-guided tracking with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and cell counting kit-8 (CCK-8) assay. Their structures were elucidated by the extensive spectroscopic analysis (1H-NMR, 13C-NMR, and two dimensions NMR), chemical derivatization, and comparison with data reported in the literature. All the isolates were evaluated for their cytotoxicity against nine cancer cell lines as well as their in vitro immunomodulatory activity. The results showed that compounds have a modest cytotoxicity toward human HeLa cell line with IC50 value below 30 μM and compounds 4 and 5 may modulate immunity to affect the growth of tumor cells. View Full-Text
Keywords: cytotoxicity-guided; phenolic derivatives; Dryopteris fragrans; chemical derivatization; immuno-regulation activity cytotoxicity-guided; phenolic derivatives; Dryopteris fragrans; chemical derivatization; immuno-regulation activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zhang, T.; Wang, L.; Duan, D.-H.; Zhang, Y.-H.; Huang, S.-X.; Chang, Y. Cytotoxicity-Guided Isolation of Two New Phenolic Derivatives from Dryopteris fragrans (L.) Schott. Molecules 2018, 23, 1652.

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