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Molecules 2018, 23(7), 1574; https://doi.org/10.3390/molecules23071574

A Novel HPLC Method for Direct Detection of Nitric Oxide Scavengers from Complex Plant Matrices and Its Application to Aloysia triphylla Leaves

1
Université Clermont Auvergne, INRA, UNH, F-63000 Clermont-Ferrand, France
2
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), Dakar-Fann PB 5005, Senegal
3
UMR IMoST 1240 Inserm, Université Clermont Auvergne, 63005 Clermont-Ferrand, France
*
Author to whom correspondence should be addressed.
Academic Editor: Alessandra Durazzo
Received: 4 June 2018 / Revised: 22 June 2018 / Accepted: 26 June 2018 / Published: 28 June 2018
(This article belongs to the Special Issue Extractable and Non-Extractable Antioxidants)
Full-Text   |   PDF [651 KB, uploaded 28 June 2018]   |  

Abstract

The present study aimed at developing an original pre-column HPLC assay allowing rapid characterization of nitric oxide (NO) scavengers from complex plant extracts. Sodium nitroprusside (SNP) was employed as a NO donor and spiked with an aqueous extract from Aloysia triphylla leaves prior to HPLC analysis. Relying on the ability of radical scavenging constituents to be oxidized upon reaction with radicals, this assay successfully allowed direct identification of three potential NO scavengers, including verbascoside, isoverbascoside, and luteolin-7-O-diglucuronide. These three phenolics were also individually assessed for their NO scavenging activities by using a Griess colorimetric assay. With respective IC50 values of 56 ± 4, 51 ± 3, and 69 ± 5 µg/mL, verbascoside, isoverbascoside, and luteolin-7-O-diglucuronide were all reported as potent NO scavenging compounds, confirming the efficiency of the SNP spiking HPLC assay. The present method can, thus, be considered as a valuable and effective approach for speeding up the discovery of NO scavenging constituents. View Full-Text
Keywords: nitric oxide scavengers; pre-column HPLC method; Aloysia triphylla; phenolics; antioxidants nitric oxide scavengers; pre-column HPLC method; Aloysia triphylla; phenolics; antioxidants
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Fraisse, D.; Degerine-Roussel, A.; Bred, A.; Ndoye, S.F.; Vivier, M.; Felgines, C.; Senejoux, F. A Novel HPLC Method for Direct Detection of Nitric Oxide Scavengers from Complex Plant Matrices and Its Application to Aloysia triphylla Leaves. Molecules 2018, 23, 1574.

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