Molecules 2018, 23(3), 611; https://doi.org/10.3390/molecules23030611
Anthocyanin-Rich Grape Pomace Extract (Vitis vinifera L.) from Wine Industry Affects Mitochondrial Bioenergetics and Glucose Metabolism in Human Hepatocarcinoma HepG2 Cells
Nathalia F. F. De Sales 1,*,†
, Leandro Silva da Costa 2, Talita I. A. Carneiro 3, Daniela A. Minuzzo 3, Felipe L. Oliveira 4, Lourdes M. C. Cabral 5, Alexandre G. Torres 1 and Tatiana El-Bacha 1,3,*
, Leandro Silva da Costa 2, Talita I. A. Carneiro 3, Daniela A. Minuzzo 3, Felipe L. Oliveira 4, Lourdes M. C. Cabral 5, Alexandre G. Torres 1 and Tatiana El-Bacha 1,3,*
1
Instituto de Química, Laboratório de Bioquímica Nutricional e de Alimentos, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941902, Brazil
2
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941902, Brazil
3
Instituto de Nutrição Josué de Castro, Laboratório de Estudos com Bioativos de Alimentos e Metabolismo Energético, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941902, Brazil
4
Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941902, Brazil
5
EMBRAPA Agroindústria de Alimentos, Guaratiba, Rio de Janeiro, RJ 21941902, Brazil
*
Authors to whom correspondence should be addressed.
†
Current Address: Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UA, UK
Received: 19 February 2018 / Revised: 5 March 2018 / Accepted: 6 March 2018 / Published: 8 March 2018
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products)
Abstract
Cancer cells demand high ATP provisions to support proliferation, and targeting of energy metabolism is a good strategy to increase their sensitivity to treatments. In Brazil, wine manufacture is expanding, increasing the amount of pomace that is produced. We determined the phenolic composition and antioxidant properties of a dark skin Grape Pomace Extract and its effects on metabolism and redox state in human hepatocarcinoma HepG2 cells. The material and the methods used represented the industrial process since pomace derived from white wine production and the extract concentrated by pilot plant scale reverse osmosis. Grape pomace extract was rich in polyphenols, mainly anthocyanins, and presented high antioxidant capacity. Short-term metabolic effects, irrespective of any cytotoxicity, involved increased mitochondrial respiration and antioxidant capacity and decreased glycolytic metabolism. Long-term incubation was cytotoxic and cells died by necrosis and GPE was not toxic to non-cancer human fibroblasts. To the best of our knowledge, this is the first report to characterize pomace extract from white wine production from Brazilian winemaking regarding its effects on energy metabolism, suggesting its potential use for pharmaceutical and nutraceutical purposes. View Full-TextKeywords:
grape pomace; anthocyanins; polyphenols; bioactivity; cancer; mitochondrial bioenergetics; glucose metabolism; in vitro; HepG2 cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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De Sales, N.F.F.; Silva da Costa, L.; Carneiro, T.I.A.; Minuzzo, D.A.; Oliveira, F.L.; Cabral, L.M.C.; Torres, A.G.; El-Bacha, T. Anthocyanin-Rich Grape Pomace Extract (Vitis vinifera L.) from Wine Industry Affects Mitochondrial Bioenergetics and Glucose Metabolism in Human Hepatocarcinoma HepG2 Cells. Molecules 2018, 23, 611.
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