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Molecules 2018, 23(12), 3359; https://doi.org/10.3390/molecules23123359

Qualitative and Quantitative Analysis of C-glycosyl-flavones of Iris lactea Leaves by Liquid Chromatography/Tandem Mass Spectrometry

1
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #24 Tongjiaxiang, Gulou District, Nanjing 210009, China
2
Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai’an 223003, Jiangsu, China
*
Authors to whom correspondence should be addressed.
Received: 23 October 2018 / Revised: 10 December 2018 / Accepted: 14 December 2018 / Published: 18 December 2018
(This article belongs to the Special Issue Qualitative and Quantitative Analysis of Bioactive Natural Products)
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Abstract

Iris lactea Pall. var. chinensis (Fisch.) Koidz. is a traditional medicinal plant resource. To make full use of the I. lactea plant resources, constituents of I. lactea leaves were determined by high performance liquid chromatography (HPLC)-quadrupole time-of-flight tandem mass spectrometry and 22 C-glycosylflavones were identified or tentatively identified. Optimal extraction of I. lactea leaves was established via single factor investigations combined with response surface methodology. Then, HPLC coupled with a diode array detector was used to quantitatively analyze the six main components of 14 batches of I. lactea leaves grown in different areas. The results showed the C-glycosylflavones were the main components of I. lactea leaves, and the total contents of detected components were relatively stable for the majority of samples. These results provide a foundation for the development and utilization of I. lactea leaves. View Full-Text
Keywords: Iris lactea Pall. var. chinensis (Fisch.) Koidz.; HPLC-Q-TOF-MS/MS; qualitative analysis; quantitative analysis; C-glycosylflavone Iris lactea Pall. var. chinensis (Fisch.) Koidz.; HPLC-Q-TOF-MS/MS; qualitative analysis; quantitative analysis; C-glycosylflavone
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Chen, D.; Meng, Y.; Zhu, Y.; Wu, G.; Yuan, J.; Qin, M.; Xie, G. Qualitative and Quantitative Analysis of C-glycosyl-flavones of Iris lactea Leaves by Liquid Chromatography/Tandem Mass Spectrometry. Molecules 2018, 23, 3359.

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