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Open AccessArticle

A Comparative Study of Advanced Stationary Phases for Fast Liquid Chromatography Separation of Synthetic Food Colorants

Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Heyrovského 1203, Hradec Králové 50005, Czech Republic
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Academic Editors: Nicola Marchetti and Anna Laura Capriotti
Molecules 2018, 23(12), 3335; https://doi.org/10.3390/molecules23123335
Received: 8 November 2018 / Revised: 9 December 2018 / Accepted: 13 December 2018 / Published: 15 December 2018
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Abstract

Food analysis demands fast methods for routine control and high throughput of samples. Chromatographic separation enables simultaneous determination of numerous compounds in complex matrices, several approaches increasing separation efficiency and speed of analysis were involved. In this work, modern types of column with monolithic rod or superficially porous particles were employed and compared for determination of eight synthetic food dyes, their chromatographic performance was evaluated. During method optimization, cyano stationary phase Chromolith Performance CN 100 × 4.6 mm and Ascentis Express ES-CN 100 × 4.6 mm, 5 µm were selected for the separation of polar colorants. The separation was performed by gradient elution of acetonitrile/methanol and 2% water solution of ammonium acetate at flow rate 2.0 mL min−1. Mobile phase composition and the gradients were optimized in order to enable efficient separation on both columns. The method using fused-core particle column provided higher separation efficiency, narrow peaks of analytes resulted in increased peak capacity and shortening of analysis time. After the validation, the method was applied for analysis of coloured beers, soft drinks and candies. View Full-Text
Keywords: monolithic column; porous shell column; food additive; dye; food colorant; chromatography; fast chromatography monolithic column; porous shell column; food additive; dye; food colorant; chromatography; fast chromatography
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Lhotská, I.; Solich, P.; Šatínský, D. A Comparative Study of Advanced Stationary Phases for Fast Liquid Chromatography Separation of Synthetic Food Colorants. Molecules 2018, 23, 3335.

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