Next Article in Journal
Fermentability of a Novel Galacto-Oligosaccharide Mixture by Lactobacillus spp. and Bifidobacterium spp.
Next Article in Special Issue
Profile of Phosphatidylserine Modifications under Nitroxidative Stress Conditions Using a Liquid Chromatography-Mass Spectrometry Based Approach
Previous Article in Journal
Synthesis of Cucurbitacin B Derivatives as Potential Anti-Hepatocellular Carcinoma Agents
Previous Article in Special Issue
A Comparative Study of Advanced Stationary Phases for Fast Liquid Chromatography Separation of Synthetic Food Colorants
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle

Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

1
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
2
Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(12), 3350; https://doi.org/10.3390/molecules23123350
Received: 30 November 2018 / Revised: 11 December 2018 / Accepted: 15 December 2018 / Published: 18 December 2018
  |  
PDF [1220 KB, uploaded 18 December 2018]
  |  

Abstract

An ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed and validated for confirmatory analysis of four nitroimidazoles and three hydroxy metabolites in honey. Honey samples were dissolved in 2% formic acid solution and nitroimidazoles and metabolites were isolated and enriched by dispersive-solid phase extraction using mixed-mode strong cation-exchange sorbent. The determination involves separation of analytes on an UHPLC C18 column and detection by multiple reaction monitoring in positive ionization mode. The recovery of the method was ranged from 90.2 to 105.6% with inter-day relative standard deviations of less than 11.2%. The limits of detection and limits of quantification were in the ranges of 0.02–0.07 µg/kg and 0.05–0.2 µg/kg, respectively. Honey samples from the market were analyzed to demonstrate the applicability of the proposed method. View Full-Text
Keywords: nitroimidazole; honey; liquid chromatography; mass spectrometry; d-SPE nitroimidazole; honey; liquid chromatography; mass spectrometry; d-SPE
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Li, X.; Ke, Y.; Wang, Y.; Wang, C.; Ye, D.; Hu, X.; Zhou, L.; Xia, X. Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry. Molecules 2018, 23, 3350.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top