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Molecules 2018, 23(12), 3244; https://doi.org/10.3390/molecules23123244

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

Department of the Sciences of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy
Author deceased on 15 February 2018.
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Author to whom correspondence should be addressed.
Academic Editors: Mingfu Wang and Yueliang Zhao
Received: 20 November 2018 / Revised: 5 December 2018 / Accepted: 6 December 2018 / Published: 7 December 2018
(This article belongs to the Special Issue Natural Products Used as Foods and Food Ingredients)
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Abstract

In the last decades, increasing demand of antioxidant-rich foods and growing interest in their putative role in prevention of degenerative diseases have promoted development of methods for measuring Antioxidant Capacity (AC). Nevertheless, most of these assays use radicals and experimental conditions far from the physiological ones, and are able to estimate only one or a few antioxidant mechanisms. On the other hand, the novel LOX/RNO and LOX–FL methods, based on secondary reactions between the soybean lipoxygenase (LOX)-1 isoenzyme and either 4-nitroso-N,N-dimethylaniline (RNO) or fluorescein (FL), may provide a more comprehensive AC evaluation. In fact, they are able to detect simultaneously many antioxidant functions (scavenging of some physiological radical species, iron ion reducing and chelating activities, inhibition of the pro-oxidant apoenzyme) and to highlight synergism among phytochemicals. They are applied to dissect antioxidant properties of several natural plant products: food-grade antioxidants, cereal and pseudocereal grains, grain-derived products, fruits. Recently, LOX–FL has been used for ex vivo AC measurements of human blood samples after short- and long-term intakes of some of these foods, and the effectiveness in improving serum antioxidant status was evaluated using the novel Antioxidant/Oxidant Balance (AOB) parameter, calculated as an AC/Peroxide Level ratio. An overview of data is presented. View Full-Text
Keywords: antioxidant activity; lipoxygenase; LOX/RNO method; LOX–FL method; ORAC; TEAC; phytochemicals; Antioxidant/Oxidant Balance; blood antioxidant status antioxidant activity; lipoxygenase; LOX/RNO method; LOX–FL method; ORAC; TEAC; phytochemicals; Antioxidant/Oxidant Balance; blood antioxidant status
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Soccio, M.; Laus, M.N.; Flagella, Z.; Pastore, D. Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview. Molecules 2018, 23, 3244.

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