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Molecules 2018, 23(12), 3189; https://doi.org/10.3390/molecules23123189

Do Osmolytes Impact the Structure and Dynamics of Myoglobin?

1
Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS), Seoul 02841, Korea
2
Department of Chemistry, Korea University, Seoul 136-713, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Chong Fang
Received: 22 October 2018 / Revised: 30 November 2018 / Accepted: 2 December 2018 / Published: 3 December 2018
(This article belongs to the Special Issue Vibrational Probes of Biomolecular Structure and Dynamics)
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Abstract

Osmolytes are small organic compounds that can affect the stability of proteins in living cells. The mechanism of osmolytes’ protective effects on protein structure and dynamics has not been fully explained, but in general, two possibilities have been suggested and examined: a direct interaction of osmolytes with proteins (water replacement hypothesis), and an indirect interaction (vitrification hypothesis). Here, to investigate these two possible mechanisms, we studied myoglobin-osmolyte systems using FTIR, UV-vis, CD, and femtosecond IR pump-probe spectroscopy. Interestingly, noticeable changes are observed in both the lifetime of the CO stretch of CO-bound myoglobin and the spectra of UV-vis, CD, and FTIR upon addition of the osmolytes. In addition, the temperature-dependent CD studies reveal that the protein’s thermal stability depends on molecular structure, hydrogen-bonding ability, and size of osmolytes. We anticipate that the present experimental results provide important clues about the complicated and intricate mechanism of osmolyte effects on protein structure and dynamics in a crowded cellular environment.
Keywords: IR spectroscopy; IR pump-probe; vibrational spectroscopy; ultrafast vibrational dynamics; vibrational probe; IR probe; myoglobin; protein dynamics; myoglobin; osmolytes IR spectroscopy; IR pump-probe; vibrational spectroscopy; ultrafast vibrational dynamics; vibrational probe; IR probe; myoglobin; protein dynamics; myoglobin; osmolytes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kossowska, D.; Kwak, K.; Cho, M. Do Osmolytes Impact the Structure and Dynamics of Myoglobin? Molecules 2018, 23, 3189.

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