Next Article in Journal
Ethanolic Extract of Folium Sennae Mediates the Glucose Uptake of L6 Cells by GLUT4 and Ca2+
Previous Article in Journal
Assessing Configurational Sampling in the Quantum Mechanics/Molecular Mechanics Calculation of Temoporfin Absorption Spectrum and Triplet Density of States
Previous Article in Special Issue
Wasabia koreana Nakai: A Preliminary Study on Nutrients and Chemical Compounds That May Impact Sensory Properties
Article Menu

Export Article

Open AccessArticle
Molecules 2018, 23(11), 2933; (registering DOI)

Extraction, Purification, and Hydrolysis Behavior of Apigenin-7-O-Glucoside from Chrysanthemum Morifolium Tea

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
National Engineering Research Center for Fruit and Vegetable Processing, Beijing 100083, China
Key Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture, Beijing 100083, China
Institute of Quality Standard & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100083, China
Author to whom correspondence should be addressed.
Received: 22 October 2018 / Revised: 3 November 2018 / Accepted: 8 November 2018 / Published: 9 November 2018
(This article belongs to the Special Issue Natural Products Used as Foods and Food Ingredients)
Full-Text   |   PDF [2137 KB, uploaded 9 November 2018]   |  


Apigenin-7-O-glucoside is an active phenolic compound in Asteraceae flowers and possesses remarkable therapeutic applications. However, its high price and low abundance in plants limit its use, meanwhile it would hydrolyze in the purification process. In this study, apigenin-7-O-glucoside extracted with ultrasound and purified with preparative HPLC from Chrysanthemum morifolium ‘Huangju’ was investigated, as well as its hydrolysis behavior and bioactivities. The optimized extraction conditions were: solid/liquid ratio: 1:20, extraction time: 35 min, temperature: 50 °C, and ultrasound power: 350 W. The content of apigenin-7-O-glucoside was up to 16.04 mg/g. Apigenin-7-O-glucoside was then purified with preparative HPLC from the extract, and confirmed by Q-TOF/MS. Apigenin-7-O-glucoside was partially hydrolyzed in acidic condition, and the hydrolysis rate depended on the pH value and temperature. The antioxidant activity increased as a result of the hydrolysis process. This study provided a green and effective way to obtain apigenin-7-O-glucoside and would be beneficial for further investigations into nutritional and functional aspects apigenin-7-O-glucoside and other glycosides. View Full-Text
Keywords: Chrysanthemum morifolium; apigenin-7-O-glucoside; purification; hydrolysis Chrysanthemum morifolium; apigenin-7-O-glucoside; purification; hydrolysis

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Wang, Y.; Xu, Z.; Huang, Y.; Wen, X.; Wu, Y.; Zhao, Y.; Ni, Y. Extraction, Purification, and Hydrolysis Behavior of Apigenin-7-O-Glucoside from Chrysanthemum Morifolium Tea. Molecules 2018, 23, 2933.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top