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Article

The Increase of Triterpene Saponin Production Induced by Trans-Anethole in Hairy Root Cultures of Panax quinquefolium

1
Pharmaceutical Biotechnology Department, Medical University of Lodz, Muszyńskiego 1, 90-151 Lodz, Poland
2
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego l, 90-151 Lodz, Poland
3
Institute of General Food Chemistry, Biotechnology and Food Science, Lodz University of Technology, Stefanowskiego St. 4/10, 90-924 Lodz, Poland
4
Department of Allergology and Respiratory Rehabilitation, Medical University of Lodz, 90-151 Lodz, Poland
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(10), 2674; https://doi.org/10.3390/molecules23102674
Received: 26 September 2018 / Revised: 15 October 2018 / Accepted: 17 October 2018 / Published: 17 October 2018
(This article belongs to the Collection Triterpenes and Triterpenoids)
In vitro cultivation is an effective way to increase pharmaceutical production. To increase ginsenoside production in hairy root cultures of American ginseng, the present study uses trans-anethole as an elicitor. The content of nine triterpene saponins was determined: Rb1, Rb2, Rb3, Rc, Rd, Rg1, Rg2, Re and Rf. Trans-anethole was found to stimulate saponin synthesis regardless of exposure time (24 and 72 h). Twenty-four hour exposure to 1 μmol trans-anethole in the culture medium resulted in the highest increase of total saponin content (twice that of untreated roots), and optimum accumulation of Rb-group saponins, with ginsenoside Rc dominating (8.45 mg g−1 d.w.). In contrast, the highest mean content of protopanaxatriol derivatives was obtained for 10 μmol trans-anethole. The Re metabolite predominated, reaching a concentration of 5.72 mg g−1 d.w.: a 3.9-fold increase over untreated roots. Elicitation with use of trans-anethole can therefore be an effective method of increasing ginsenoside production in shake flasks. View Full-Text
Keywords: hairy root cultures; trans-anethole; elicitation hairy root cultures; trans-anethole; elicitation
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MDPI and ACS Style

Kochan, E.; Szymczyk, P.; Kuźma, Ł.; Szymańska, G.; Wajs-Bonikowska, A.; Bonikowski, R.; Sienkiewicz, M. The Increase of Triterpene Saponin Production Induced by Trans-Anethole in Hairy Root Cultures of Panax quinquefolium. Molecules 2018, 23, 2674. https://doi.org/10.3390/molecules23102674

AMA Style

Kochan E, Szymczyk P, Kuźma Ł, Szymańska G, Wajs-Bonikowska A, Bonikowski R, Sienkiewicz M. The Increase of Triterpene Saponin Production Induced by Trans-Anethole in Hairy Root Cultures of Panax quinquefolium. Molecules. 2018; 23(10):2674. https://doi.org/10.3390/molecules23102674

Chicago/Turabian Style

Kochan, Ewa, Piotr Szymczyk, Łukasz Kuźma, Grażyna Szymańska, Anna Wajs-Bonikowska, Radosław Bonikowski, and Monika Sienkiewicz. 2018. "The Increase of Triterpene Saponin Production Induced by Trans-Anethole in Hairy Root Cultures of Panax quinquefolium" Molecules 23, no. 10: 2674. https://doi.org/10.3390/molecules23102674

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