Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient
by
Yong Zhou 1,†, Li-Rong Han 1,2,†, Hong-Wei He 1, Bu Sang 3, Dai-Lin Yu 3, Jun-Tao Feng 1,2 and Xing Zhang 1,2,*
Yong Zhou 1,†, Li-Rong Han 1,2,†, Hong-Wei He 1, Bu Sang 3, Dai-Lin Yu 3, Jun-Tao Feng 1,2 and Xing Zhang 1,2,*
1
Research and Development Center of Biorational Pesticides, Northwest Agriculture & Forestry University, Yangling 712100, Shaanxi, China
2
Shaanxi Research Center of Biopesticide Engineering & Technology, Yangling 712100, Shaanxi, China
3
Agriculture Research Institute, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa 850032, Tibet, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2018, 23(1), 125; https://doi.org/10.3390/molecules23010125
Received: 8 December 2017 / Revised: 26 December 2017 / Accepted: 5 January 2018 / Published: 11 January 2018
(This article belongs to the Collection Phytochemicals: Biosynthesis, Metabolism and Biological Activities)
The effects of temperature, agitation and aeration on glycoprotein GP-1 production by Streptomyces kanasenisi ZX01 in bench-scale fermentors were systematically investigated. The maximum final GP-1 production was achieved at an agitation speed of 200 rpm, aeration rate of 2.0 vvm and temperature of 30 °C. By using a dynamic gassing out method, the effects of agitation and aeration on volumetric oxygen transfer coefficient (kLa) were also studied. The values of volumetric oxygen transfer coefficient in the logarithmic phase increased with increase of agitation speed (from 14.53 to 32.82 h−1) and aeration rate (from 13.21 to 22.43 h−1). In addition, a successful scale-up from bench-scale to pilot-scale was performed based on volumetric oxygen transfer coefficient, resulting in final GP-1 production of 3.92, 4.03, 3.82 and 4.20 mg/L in 5 L, 15 L, 70 L and 500 L fermentors, respectively. These results indicated that constant volumetric oxygen transfer coefficient was appropriate for the scale-up of batch fermentation of glycoprotein GP-1 by Streptomyces kanasenisi ZX01, and this scale-up strategy successfully achieved 100-fold scale-up from bench-scale to pilot-scale fermentor.
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MDPI and ACS Style
Zhou, Y.; Han, L.-R.; He, H.-W.; Sang, B.; Yu, D.-L.; Feng, J.-T.; Zhang, X. Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient. Molecules 2018, 23, 125.
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