Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient

Zhou, Yong; Han, Li-Rong; He, Hong-Wei; Sang, Bu; Yu, Dai-Lin; Feng, Jun-Tao; Zhang, Xing

doi:10.3390/molecules23010125

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Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient

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Research and Development Center of Biorational Pesticides, Northwest Agriculture & Forestry University, Yangling 712100, Shaanxi, China

Shaanxi Research Center of Biopesticide Engineering & Technology, Yangling 712100, Shaanxi, China

Agriculture Research Institute, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa 850032, Tibet, China

Author to whom correspondence should be addressed.

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These authors contributed equally to this work.

Molecules 2018, 23(1), 125; https://doi.org/10.3390/molecules23010125

Received: 8 December 2017 / Revised: 26 December 2017 / Accepted: 5 January 2018 / Published: 11 January 2018

(This article belongs to the Collection Phytochemicals: Biosynthesis, Metabolism and Biological Activities)

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Abstract

The effects of temperature, agitation and aeration on glycoprotein GP-1 production by Streptomyces kanasenisi ZX01 in bench-scale fermentors were systematically investigated. The maximum final GP-1 production was achieved at an agitation speed of 200 rpm, aeration rate of 2.0 vvm and temperature of 30 °C. By using a dynamic gassing out method, the effects of agitation and aeration on volumetric oxygen transfer coefficient (k_La) were also studied. The values of volumetric oxygen transfer coefficient in the logarithmic phase increased with increase of agitation speed (from 14.53 to 32.82 h⁻¹) and aeration rate (from 13.21 to 22.43 h⁻¹). In addition, a successful scale-up from bench-scale to pilot-scale was performed based on volumetric oxygen transfer coefficient, resulting in final GP-1 production of 3.92, 4.03, 3.82 and 4.20 mg/L in 5 L, 15 L, 70 L and 500 L fermentors, respectively. These results indicated that constant volumetric oxygen transfer coefficient was appropriate for the scale-up of batch fermentation of glycoprotein GP-1 by Streptomyces kanasenisi ZX01, and this scale-up strategy successfully achieved 100-fold scale-up from bench-scale to pilot-scale fermentor. View Full-Text

Keywords: scale-up; glycoprotein; Streptomyces; anti-TMV; volumetric oxygen transfer coefficient

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MDPI and ACS Style

Zhou, Y.; Han, L.-R.; He, H.-W.; Sang, B.; Yu, D.-L.; Feng, J.-T.; Zhang, X. Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient. Molecules 2018, 23, 125. https://doi.org/10.3390/molecules23010125

AMA Style

Zhou Y, Han L-R, He H-W, Sang B, Yu D-L, Feng J-T, Zhang X. Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient. Molecules. 2018; 23(1):125. https://doi.org/10.3390/molecules23010125

Chicago/Turabian Style

Zhou, Yong, Li-Rong Han, Hong-Wei He, Bu Sang, Dai-Lin Yu, Jun-Tao Feng, and Xing Zhang. 2018. "Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient" Molecules 23, no. 1: 125. https://doi.org/10.3390/molecules23010125

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Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient

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