Next Article in Journal
Functional Analysis of a Wheat AGPase Plastidial Small Subunit with a Truncated Transit Peptide
Next Article in Special Issue
Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures
Previous Article in Journal
Under-Reported Aspects of Platinum Drug Pharmacology
Communication

In Vitro Selection of DNA Aptamers that Binds Geniposide

1
School of Basic Medical Science, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China
2
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada
3
Beijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China
*
Author to whom correspondence should be addressed.
Academic Editor: Masayasu Kuwahara
Molecules 2017, 22(3), 383; https://doi.org/10.3390/molecules22030383
Received: 26 January 2017 / Accepted: 21 February 2017 / Published: 28 February 2017
(This article belongs to the Special Issue Nucleic Acid Aptamers)
Geniposide is a key iridoid glycoside from Gardenia jasminoides fructus widely used in traditional Chinese herbal medicine. However, detection of this small molecule represents a significant challenge mostly due to the lack of specific molecular recognition elements. In this study, we have performed in vitro selection experiments to isolate DNA aptamers that can specifically bind geniposide. Using a stringent selection procedure, we have isolated DNA aptamers that can distinguish geniposide from genipin and glucose, two structural analogs of geniposide. Two top aptamers exhibit low micromolar binding affinity towards geniposide, but show significantly reduced affinity to genipin and glucose. These aptamers have the potential to be further developed into analytical tools for the detection of geniposide. View Full-Text
Keywords: geniposide; aptamer; in vitro selection; DNA; Chinese herbal medicine; molecular recognition geniposide; aptamer; in vitro selection; DNA; Chinese herbal medicine; molecular recognition
Show Figures

Figure 1

MDPI and ACS Style

Zhang, A.; Chang, D.; Zhang, Z.; Li, F.; Li, W.; Wang, X.; Li, Y.; Hua, Q. In Vitro Selection of DNA Aptamers that Binds Geniposide. Molecules 2017, 22, 383. https://doi.org/10.3390/molecules22030383

AMA Style

Zhang A, Chang D, Zhang Z, Li F, Li W, Wang X, Li Y, Hua Q. In Vitro Selection of DNA Aptamers that Binds Geniposide. Molecules. 2017; 22(3):383. https://doi.org/10.3390/molecules22030383

Chicago/Turabian Style

Zhang, Aozhe; Chang, Dingran; Zhang, Zijian; Li, Fan; Li, Weihong; Wang, Xu; Li, Yingfu; Hua, Qian. 2017. "In Vitro Selection of DNA Aptamers that Binds Geniposide" Molecules 22, no. 3: 383. https://doi.org/10.3390/molecules22030383

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop